我国不同地区麦蚜携带BYDV-GAV比率的差异

为了研究我国不同地区麦蚜携带大麦黄矮病毒麦二叉蚜麦长管蚜非专化性株系(BYDV GAV)比率的差异,采用RT-PCR技术,对BYDV-GAV的传毒介体麦蚜带毒情况进行检测.所用方法具有较高的灵敏度和特异性,测定样本用量可少至1/200头蚜虫；对采自我国主要麦区的蚜虫样本进行分子检测,山西、甘肃、青海、陕西11个小麦黄矮病重病区蚜虫样本带毒率为56％～91.5％,而河北、河南两省4个非重病区蚜虫样本带毒率为2.5％～33％.通过试验证实,我国不同地区麦蚜携带BYDV-GAV比率存在差异,小麦黄矮病重病区山西、甘肃、青海、陕西等地的麦蚜带毒率高,而非重病区河北、河南等地的麦蚜带毒率低.

Difference in the proportion of aphids carrying Barley yellow dwarf virus-GAV between different areas in china

Difference in the proportion of aphids carrying Barley yellow dwarf virus-GAV between different areas in China was studied.The situation of aphids carrying BYDV-GAV was surveyed by using RT-PCR assay.The method was very sensitive and specific,and the virus in only 1/200 of an aphid could be detected.Aphids collected from major wheat-growing regions in China were tested for the proportion of aphids carrying virus.The proportion of aphids carrying virus was ranged from 56% to 91.5%.They were collected from 11 seriously affected areas in Shanxi,Gansu,Qinghai and Shaanxi provinces,while 2.5%—33% from 4 non-seriously affected areas in Henan and Hebei provinces.Our study confirmed that there was difference in the proportion of aphids carrying BYDV-GAV among different areas of China.The proportions of aphids carrying virus were high in seriously-affected areas like Shanxi,Gansu,Qinghai and Shaanxi provinces,while low in non-seriously affected areas like Henan and Hebei provinces.