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钙对弱光胁迫下番茄叶片保护酶活性及可溶性蛋白含量的影响
引用本文:李天来,李益清.钙对弱光胁迫下番茄叶片保护酶活性及可溶性蛋白含量的影响[J].园艺学报,2008,35(11):1601-1606.
作者姓名:李天来  李益清
作者单位:(沈阳农业大学园艺学院,辽宁省设施园艺重点实验室,沈阳 110161)
摘    要: 为明确Ca2+对番茄耐弱光特性的调控作用,以番茄(Lycopersicon esculentum Mill)耐弱光品系W为试材,研究了弱光(50%自然光强)胁迫条件下,钙和钙加IAA处理对叶片保护酶活性以及可溶性蛋白质含量的调控作用。结果表明:弱光胁迫下(实测光通量密度为556.8~656.6μmol·m-2·s-1)27mmol·L-1的CaCl2处理可显著降低番茄叶片MDA含量,提高SOD、CAT、POD的活性及可溶性蛋白含量,使这些指标达到正常光照下的对照植株水平;IAA进一步增强了Ca2+的这些作用。Ca2+的这些作用可能是其提高番茄植株弱光耐受性、防止植株弱光伤害的重要原因之一。

关 键 词:番茄    弱光胁迫  保护酶活性  可溶性蛋白
收稿时间:2008-05-22

Effects of Calcium on Activities of Protective Enzymes and Soluble Protein Content in the Leaves of Tomato under Low Light Stress
LI Tian-lai,LI Yi-qing
.Effects of Calcium on Activities of Protective Enzymes and Soluble Protein Content in the Leaves of Tomato under Low Light Stress[J].Acta Horticulturae Sinica,2008,35(11):1601-1606.
Authors:LI Tian-lai  LI Yi-qing
Institution:(College of Horticulture, Shenyang Agricultural University, Liaoning Province Key Laboratory of Protected Horticulture, Shenyang 110161, China)
Abstract:In order to investigate the regulating mechanism of calcium on tomato low light tolerance, leaves of one tomato low light tolerance strain W (Lycopersicon esculentum Mill) were used to study the changes of protective enzymes activities and soluble protein content in leaves after the tomato were treated by calcium or calcium and IAA under the 50% nature light intensity (identical radiant flux of 556.8 to 656.6 μmol·m-2·s-1). The results showed that 27 mmol·L-1 CaCl2 treatment could significantly decrease MDA content, increase the activities of SOD,CAT,and POD, promote the content of soluble protein in the leaves of tomato under low light stress reach the control level under 100% nature light intensity; auxin could promote the effect of Ca2+ further. These results indicated that the effects of calcium on the activities of protective enzymes and the content of soluble protein in the leaves of tomato under low light stress maybe one of the important reasons for that calcium could increased low light tolerance of tomato and prevented low light damage to the plant.
Keywords:tomato  calcium  low light stress  activities of cell defense enzymes  soluble protein
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