| 基于5.8S rDNA和ITS序列探讨亚洲瓠瓜的地理分化 |
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| 引用本文： | 周先治,陈阳,陈晟,吴宇芬,赵依杰.基于5.8S rDNA和ITS序列探讨亚洲瓠瓜的地理分化[J].中国蔬菜,2011,1(6):49-53. |
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| 作者姓名： | 周先治 陈阳 陈晟 吴宇芬 赵依杰 |
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| 作者单位： | 1.福建省农业科学院农业生物资源研究所，福建福州 350003；2福州市农业科学研究所，福建福州 350018 |
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| 摘 要： | 基于5.8S rDNA及ITS序列对23份来自中国、泰国和日本的亚洲瓠瓜品种进行了地理分化研究。结果发现，23份瓠瓜材料的ITS1+5.8S rDNA+ITS2序列的长度在611～627 bp之间，G+C含量在54.17 %～63.26 %之间，信息位点数65个，占总碱基数的11.09 %；源自日本的2份瓠瓜品种的序列长度短于其他21份瓠瓜品种，G+C含量也明显低于其他瓠瓜品种。从5.8S rDNA及ITS序列构建的系统树可以看出，源自日本的瓠瓜品种与源自中国和泰国的瓠瓜品种存在地理分化现象，分属于不同的分支，支持率达到99 %。序列间的同质性检验结果显示，2份源自日本的瓠瓜品种与源自中国和泰国的瓠瓜品种差异显著。
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| 关 键 词： | 5.8S rDNA 亚洲瓠瓜 内转录间隔区 地理分化 |
| 收稿时间： | 2010-11-19; |
Studiesy on Geographic Divergence of Lagenaria siceraria ssp.asiatica Based on 5.8S rDNA and ITS Sequence Data |
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| ZHOU Xian-zhi,CHEN Yan,CHEN Sheng,WU Yu-fen,ZHAO Yi-jie.Studiesy on Geographic Divergence of Lagenaria siceraria ssp.asiatica Based on 5.8S rDNA and ITS Sequence Data[J].China Vegetables,2011,1(6):49-53. |
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| Authors: | ZHOU Xian-zhi CHEN Yan CHEN Sheng WU Yu-fen ZHAO Yi-jie |
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| Institution: | 1.Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, Fujian, China;2.Fuzhou Institute of Agricultural Sciences, Fuzhou 350018, Fujian, China |
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| Abstract: | The geographic divergence of the Lagenaria siceraria ssp.asiatica were examined using ITS1+5.8S rDNA+ITS2 sequences data.The sequences were obtained from 23 species of L.siceraria ssp.asiatica from China,Thailand and Japan.The results showed that within L.siceraria ssp.asiatica,ITS1+5.8S rDNA+ITS2 was 611-627 bp,the G+C content of the sequences was 54.17 %-63.26 %,65 informative sites(11.09 %)within L.siceraria ssp.asiatica are found in the ITS1+5.8S rDNA+ITS2 sequences.The length of the ITS1+5.8S rDNA+ITS2... |
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| Keywords: | 5 8S rDNA |
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