| 重组木聚糖酶生产低聚木糖的实验研究 |
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| 引用本文： | 欧阳嘉,刘明,李鑫,陈牧,连之娜.重组木聚糖酶生产低聚木糖的实验研究[J].林产化学与工业,2011,31(2):37-42. |
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| 作者姓名： | 欧阳嘉 刘明 李鑫 陈牧 连之娜 |
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| 作者单位： | 南京林业大学化学工程学院,江苏南京,210037 |
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| 基金项目： | 国家自然科学基金资助项目(31070513); 国家重点基础研究发展计划(973)资助项目(2007CB707801); 江苏省高校自然科学研究重大项目(10KJA22019) |
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| 摘 要： | 研究了重组木聚糖酶C0602生产低聚木糖的制备工艺,考察了不同类型底物、底物浓度和酶用量对酶解效率和低聚木糖产率的影响.实验结果表明,低聚木糖生产原料以抽提木聚糖为宜.桦木木聚糖质量浓度30g/L,重组木聚糖酶用量20 IU/g条件下水解4 h,酶解率可达56.05%,低聚木糖产率(C2～C6)为29.76%,产品平均...
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| 关 键 词： | 木聚糖酶 低聚木糖 酶水解 聚合度 |
Experimental Investigation of Xylooligosaccharides Production by Recombinant Xylanase |
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| OUYANG Jia,LIU Ming,LI Xin,CHEN Mu,LIAN Zhi-na.Experimental Investigation of Xylooligosaccharides Production by Recombinant Xylanase[J].Chemistry & Industry of Forest Products,2011,31(2):37-42. |
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| Authors: | OUYANG Jia LIU Ming LI Xin CHEN Mu LIAN Zhi-na |
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| Institution: | OUYANG Jia,LIU Ming,LI Xin,CHEN Mu,LIAN Zhi-na (The College of Chemical Engineering,Nanjing Forestry University,Nanjing 210037,China) |
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| Abstract: | Xylooligosaccharides(XOs) production using the recombinant xylanase C0602 was studied.Effects of different substrates,substrate concentration and enzyme dosage on the enzymatic hydrolysis and xylooligosaccharide yield were investigated.The results showed that the extracted xylan was the suitable substrate for xylooligosaccharides production.When 30 g/L birch wood xylan was treated with the dosage of 20 U/g xylanase for hydrolysis 4 h,the hydrolysis yield was 56.05 % and the xylooligosaccharide yield(C2-C6) ... |
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| Keywords: | xylanase xylooligosaccharide enzymatic hydrolysis degree of polymerization |
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