止咳平喘颗粒的质量标准研究简

为建立止咳平喘颗粒的质量标准，本试验采用薄层色谱法（TLC）对组方中紫菀、桑白皮、百部、枇杷叶及鱼腥草进行定性鉴别，采用高效液相色谱法测定本方中紫菀酮的含量。结果显示，TLC鉴别专属性强，斑点清晰；采用C₁₈反相柱，以乙腈—水为流动相，检测波长为200 nm，其线性范围为10.2～510.0 μg/mL，回归方程为A＝11481C＋36617（R²＝1，n=6）。平均回收率为99.89%，RSD=1.57%。本试验所建立的方法简便、准确、专属性强、重复性好，可有效地控制颗粒的质量。

Study on Quality Standard of Zhike Pingchuan Granules

To establish a quality standard for Zhike Pingchuan granules, Atarian aster, Cortex mori radices, Radix stemonae, Loquat leaf and Houttuynia in the components were identified by TLC, and the content of Shionone was detected by HPLC.The results showed that the TLC identification was highly specific and the spot was clear; the HPLC separation was carried out on C₁₈ column, the mixture of acetonitrile-water was used as mobile phase. The detection wavelength was 200 nm, the calibration curve of Shionone showed a good linearity over the range of 10.2 to 510.0 μg/mL, the regression equation was A＝11481C＋36617(R²＝1,n=6), the average recovery was 99.89%, RSD=1.57%. The method established by this experiment was simple, accurate and focused with good reproducibility, so it could be used to control the quality of the preparation effectively.