基于蛋白质组解析大球盖菇多糖促进猕猴桃生长及抗高温的分子机制

为明确大球盖菇多糖施用对猕猴桃生长及抗性诱导的作用和机制,本研究以红阳猕猴桃为试验材料,通过叶片形态结构、生理生化指标及蛋白质组学分析,研究大球盖菇多糖对猕猴桃的影响。结果表明,大球盖菇多糖处理后猕猴桃叶面积增加、叶片变厚,叶绿素含量增加,叶片栅栏组织和海绵组织排列紧密、厚度增加。高温胁迫条件下,处理植株叶片的叶绿素荧光Fv/Fm比值、过氧化物酶活性和多酚氧化酶活性显著高于对照。利用非标记定量蛋白质组学技术共筛选出326个差异蛋白,其中上调蛋白166个(显著上调1.5倍),下调蛋白160个(显著下调1.5倍)。通过基因功能分类和代谢通路富集等生物信息学分析发现,大球盖菇多糖对猕猴桃叶绿体蛋白、胁迫应答、氧化还原相关蛋白表达的影响最大,对光合作用、激素信号途径、次生代谢产物生物合成等代谢途径改变最明显。本研究结果为多糖类抗性诱导剂的开发和利用提供了新资源和理论基础。

Proteomic Analysis Reveals Mechanisms of Stropharia rugosoannulata Polysaccharides Promoting Kiwifruit Growth and High Temperature Resistance

In order to assess the effect of Stropharia rugosoannulata polysaccharides(SPS)on the growth and induced resistance of kiwifruit and unravel the regulation mechanism,leaf morphology and structure,physiological and proteomic analysis were carried out with the variety Hongyang.Our results showed that compared with the control,the area,thickness and chlorophyll content of its leaves increased after treatment with SPS.The cross section of blade showed that the palisade tissue and spongy tissue were closely arranged and thickened after being sprayed with SPS.Under high temperature stress,the chlorophyll fluorescence Fv/Fm ratio,peroxidase and polyphenoloxidase activity of the treated leaves increased significantly.A total of 326 differentially accumulated proteins(DAPs)were identified by label-free quantitative proteomics,including 166 up-regulated proteins(up-regulated by 1.5-fold and P<0.05)and 160 down-regulated proteins(down-regulated by 1.5-fold and P<0.05).Gene ontology annotation revealed that SPS dramatically changed the levels of proteins related to chloroplast,stress response and redox and the pathways involved in photosynthesis,hormone signaling and biosynthesis of secondary metabolites.S.rugosoannulata polysaccharides may improve photosynthetic efficiency and promote the growth of kiwifruit leaves via regulating the expression of proteins related to photosynthesis.The regulation on stress response and redox related proteins indicated that SPS may have a potential role in inducing system resistance of kiwifruit.Our results provide new resources and a theoretical basis for the development and utilization of polysaccharide resistance inducers.