莲藕采后主要致腐真菌分离鉴定及生物学特性研究

为明确莲藕采后主要致腐真菌的种类,本研究采用组织分离法对在25℃贮藏条件下自然腐烂的莲藕进行病原菌分离纯化及致病性检测,对主要致腐真菌进行形态学及分子生物学鉴定,并研究其生物学特性.结果表明,从常温下自然腐烂的莲藕中分离出LO-1、LO-2、LO-3共3种真菌;回接试验结果表明,LO-1是主要致腐菌,经鉴定为可可毛色二...

Identification and Biological Characteristics of Dominant Spoilage Fungi in Postharvest Lotus Roots

To elucidate the dominant pathogenic fungi of postharvest lotus roots,the pathogens were isolated and purified from the naturally rotten samples stored at 25℃.The dominant pathogenic strains were identified by morphological and molecular biology assay,and their biological characteristics were investigated.Three fungi named LO-1,LO-2,LO-3 were isolated from the naturally decomposed lotus roots,and LO-1,identified as Lasiodiplodia theobromae,was the dominant pathogenic fungus by pathogenicity tests.The optimal temperature for mycelial growth of L.theobromae was 25℃and continuous light could promote the mycelium growth,while the lethal temperature was 55℃for 10 min.The strain could grow at pH 4~10 and the largest expansion diameter was at pH 5.The optimal carbon and nitrogen source for L.theobromae growth was sucrose and yeast extract.The largest expansion diameter of mycelia was measured on lotus root juice-glucose media.The oatmeal media and continuous light could promote the spore production of L.theobromae.This study would set up a targeted technical guidance for the preservation and prolongation of the storage time of lotus roots.