浙江省广西越桔组织培养及遗传多样性分析

为了保育浙江省广西越桔,以凤阳山的广西越桔未成熟果实为材料,开展了未成熟种子培养、离体快繁体系建立、种群增强与回归和遗传多样性分析等研究。结果表明,接种至发育培养基(WPM+活性炭2.0 g·L^-1+谷氨酰胺0.4 g·L^-1+酶水解酪蛋白0.5 g·L^-1)后,85.19%未成熟种子发育完全,接种至萌发培养基(WPM+6-BA 1.0 mg·L^-1+GA₃ 1.5 mg·L^-1)后,30.43%发育完全的种子萌发,总萌发率为25.92%。最适增殖培养基为6号增殖培养基(WPM+ZT 0.2 mg·L^-1+NAA 0.5 mg·L^-1),增殖系数达106.67%,在生根培养基(WPM+IBA 1.0 mg·L^-1)上培养45 d后生根率为100%。种群增强与回归过程中植株长势良好,株高及株幅明显增加,部分植株与原生株及其他回归植株相比出现明显表型差异。遗传多样性分析结果显示,原生株与后代群体间的遗传相似系数在0.727 1~0.977 8之间,平均相似系数为0.841 0,极差为0.250 7。本研究成果可为浙江省广西越桔及相近处境珍稀植物的保育和利用提供理论与实践依据。

Tissue Culture and Genetic Analysis of Vaccinium sinicum in Zhejiang Province

In order to conserve Vaccinium sinicum in Zhejiang Province,the immature fruits were selected to conduct in vitro immature seed culture,in vitro rapid propagation,population tein forcement and reintroduction and genetic diversity analysis.The results showed that 85.19%of immature seeds were full developed when inoculated on the development medium(WPM+activated carbon 2.0 g·L^-1+glutamine 0.4 g·L^-1+enzymatic hydrolyzed casein 0.5 g·L^-1).After inoculated on the germination medium(WPM+6-BA 1.0 mg·L^-1+GA₃1.5 mg·L^-1),30.43%of fully developed seeds germinated,and the total germination rate was 25.92%.The NO.6 propagation medium(WPM+ZT 0.2 mg·L^-1+NAA 0.5 mg·L^-1)was the best for inducing of regeneration buds with the proliferation rate of 106.67%.After being cultured on the rooting medium(WPM+IBA 1.0 mg·L^-1)for 45 days,the rooting rate reached 100%.During the population reinforcement and reintroduction,the plants grew well,with its height and width increasing significantly,and some of which showed significant differences in phenotype from the original plant and the other reintroduced plants.The results of genetic diversity analysis showed that the genetic similarity coefficient between the original plant and the progeny population ranged from 0.7271 to 0.9778,the average similarity coefficient was 0.8410,and the range was 0.2507.The study provides theoretical and practical basis for the conservation and utilization of V.sinicum in Zhejiang Province and other rare plants.