利用分子标记辅助选择Pigm-1基因改良恢复系R20的稻瘟病抗性

水稻稻瘟病是水稻的重要病害之一,给我国甚至全世界的水稻安全生产带来了巨大隐患.选育优质抗稻瘟病恢复系,进而培育稻瘟病水稻杂交组合,是选育抗稻温病品种的有效途径之一.本研究以双抗77009为抗性基因Pigm-1的供体亲本,以感病恢复系R20为受体亲本,通过杂交和连续回交方法,并利用分子标记辅助选择技术,定向改良优质水稻恢...

Improving Blast Resistance of R20 by Molecular Marker-Assisted Selection of Pigm-1 Gene

Rice blast is one of the most important rice diseases,which brings great hidden danger to the safety and production of rice in China and even the whole world.Developing functional markers of broad-spectrum R gene combined with molecular marker assisted selection is one of the most effective methods to improve blast resistance of rice.In this study,Shuangkang 77009 was used as the donor parent of the resistance gene Pigm-1,and the susceptible restorer line R20 was used as the recipient parent.Through hybridization,backcross,and molecular marker assisted selection technology,four improved lines of R20 with enhanced blast resistance were obtained.Indoor inoculation and field naturally induced identification showed that the improved lines R20-1,R20-2,R20-3 and R20-4 were resistant to rice blast.Background restoration analysis showed that the four improved lines basically restored the background of the recipient parent.Compared with R20,the agronomic traits of R20-1,R20-2 and R20-3 showed no significant differences in plant height,panicle length,effective panicle,spikelet number per panicle,seed setting rate,grain length,grain width and thousand seeds weight.However,the grain length and thousand seeds weight of R20-4 were significantly increased compared to those of R20.This study indicated that the utilization of molecular marker assisted selection technology not only improved the resistance of the restorer R20,but also increased the yield in R20-4.This study provides some value and reference for molecular breeding of rice blast resistance gene Pigm-1.