| 山葡萄种内遗传多样性的SSR分析 |
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| 引用本文： | 吴子龙,王军,沈育杰,路文鹏.山葡萄种内遗传多样性的SSR分析[J].果树学报,2008,25(6):821-827. |
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| 作者姓名： | 吴子龙 王军 沈育杰 路文鹏 |
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| 作者单位： | [1]东北林业大学林学院,哈尔滨150040 [2]东北林业大学林木遗传育种与生物技术教育部重点实验室,哈尔滨150040 [3]中国农业科学院特产研究所,吉林132109 |
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| 基金项目： | 国家林业局动植物保护基金，黑龙江省博士后科研启动基金 |
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| 摘 要： | 利用26对SSR引物对124份山葡萄种质资源的遗传多样性初步分析,等位基因(Na)数平均为3.6923,有效等位基因(Ne)数平均为2.5779,Shannon多样性指数(I)平均为1.0195,基因多样度(Nei)平均为0.5762。野生种质资源的遗传多样性高于选育品种;而野生种质资源中黑龙江省的遗传多样性高,其次是吉林省野生资源,辽宁省野生资源最低。山葡萄种质的遗传相似性系数J为0.224～0.972,平均遗传相似系数为0.588。根据遗传相似性系数进行UPGMA聚类,建立树状聚类图。利用26对SSR标记能将供试的124份山葡萄种质相互区分,在遗传相似性系数0.39处将供试山葡萄种质资源分为6大类群,多数种质的聚类和山葡萄长期生长发育的地理分析研究结果一致。
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| 关 键 词： | 山葡萄 SSR 遗传多样性 聚类分析 |
Analysis of genetic diversity of Vitis amurensis by using SSR markers |
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| WU Zi-long,WANG Jun,SHEN Yu-jie,LU Wen-peng.Analysis of genetic diversity of Vitis amurensis by using SSR markers[J].Journal of Fruit Science,2008,25(6):821-827. |
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| Authors: | WU Zi-long WANG Jun SHEN Yu-jie LU Wen-peng |
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| Institution: | WU Zi-long1,WANG Jun1,2,SHEN Yu-jie3,LU Wen-peng3(1College of Forestry,Northeast Forestry University,2Key Laboratory of Forest Tree Genetic Improvement , Biotechnology,Ministry of Education,Harbin,Heilongjiang 150040 China,3Institute of Special Wild Economic Animals , Plants,CAAS,Jilin 132109 China) |
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| Abstract: | Twenty-six pairs of SSR molecular markers were used to analyze the genetic diversity of 124 Vitis amurensis germplasms.The preliminary results indicated that the average allelic gene number(Na) was 3.692 3,the effective allelic number(Ne) was 2.5779,and Shannon Index of diversity(I) was 1.0195,and the average expected heterozygousity(Nei) was 0.576 2.The genetic diversity of wild germplasms is higher than cultivars;the genetic diversity in Heilongjiang is the high-est,Jilin is the next,and Liaoning is the l... |
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| Keywords: | SSR |
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