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| 利用SSR分析标记鉴定大豆育种中杂交真伪和后代系谱 | |
| 引用本文： | 韩冬伟,刘春燕,胡振帮,蒋洪蔚,于凤瑶,范冬梅,郭广宇,孙蕊,赵曦,曲忠诚,胡国华,陈庆山.利用SSR分析标记鉴定大豆育种中杂交真伪和后代系谱[J].东北农业大学学报,2012(10):91-95. |
| 作者姓名： | 韩冬伟 刘春燕 胡振帮 蒋洪蔚 于凤瑶 范冬梅 郭广宇 孙蕊 赵曦 曲忠诚 胡国华 陈庆山 |
| 作者单位： | 1. 东北农业大学农学院,哈尔滨 150030 2. 黑龙江省农业科学院齐齐哈尔分院,黑龙江 齐齐哈尔 161006 3. 黑龙江省农垦科研育种中心,哈尔滨 150090 4. 黑龙江省农业科学院生物技术研究所,哈尔滨 150086 5. 国家大豆工程技术研究中心,哈尔滨 150050 |
| 基金项目： | 国家转基因专项(2009ZX08009-013B);现代农业产业技术体系大豆产业体系(200903003) |
| 摘 要： | 研究通过筛选亲本间具有多态性的SSR引物,对材料的亲本和后代进行分子鉴定,获得材料间的系谱关系.供试的23份材料通过分子鉴定得到:4份后代材料可能为伪杂种后代;1对基因型相同的姊妹系;另外确定12份后代材料可能的一个亲本材料和1份后代材料的两个可能亲本.研究结果表明,在亲本基因型纯合的情况下,采用分子标记有助于大豆育种的系谱分析与鉴定. |
| 关 键 词： | 大豆 育种 系谱 分子鉴定 |
Identification of true or pseudo hybrid and offspring pedigree in soybean breeding by SSR markers |
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| HAN Dongwei,LIU Chunyan,HU Zhenbang,JIANG Hongwei,YU Fengyao,FAN Dongmei,GUO Guangyu,SUN Rui,ZHAO Xi,QU Zhongcheng,HU Guohua,CHEN Qingshan.Identification of true or pseudo hybrid and offspring pedigree in soybean breeding by SSR markers[J].Journal of Northeast Agricultural University,2012(10):91-95. | |
| Authors: | HAN Dongwei LIU Chunyan HU Zhenbang JIANG Hongwei YU Fengyao FAN Dongmei GUO Guangyu SUN Rui ZHAO Xi QU Zhongcheng HU Guohua CHEN Qingshan |
| Institution: | 1(1.School of Agriculture,Northeast Agricultural University,Harbin 150030,China;2.Qiqihar Sub-academy of Heilongjiang Academy of Agricultural Sciences,Qiqihar Heilongjiang 161006,China;3.The Crop Research and Breeding Center of Heilongjiang Land-Reclamation,Harbin 150090,China;4.Institute of Biotechnology Research,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;5.The National Research Center of Soybean Engineering and Technology,Harbin 150050,China) |
| Abstract: | In order to solve practical problems in soybean breeding,a set of SSR primer were used to screen the polymorphism.Then 23 tested materials were genotyped to reveal their relationship.The results showed that four lines were pseudo-hybrid;one pair of lines were potential sister lines with exactly the same genotypic profiles;12 lines were carrying one parental genetic background and one had both parental backgrounds.Therefore,molecular markers should be useful in detecting the soybean crossing or pedigree relationship. |
| Keywords: | soybean breeding pedigree molecular identification |
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