茯砖茶对H2O2诱发LLC-PK1细胞损伤的保护作用

探讨茯砖茶乙醇提取物(FTE)对H2O2诱发猪肾近曲小管LLC-PK1上皮细胞氧化损伤的保护作用.以不同质量浓度(10～200 μg/mL)的FTE预培养LLC-PK1细胞24 h后,换用含500 μmol/L H2O2的DMEM细胞培养液继续培养4h建立细胞氧化损伤模型.MTT法检测细胞生存率,硫代巴比妥酸比色法测定细胞内丙二醛(malondialdehyde,MDA)含量,比色法测定细胞内过氧化氢酶(catalase,CAT),超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-px)含量.经不同浓度FTE预处理24 h后,受损细胞的生存率上升,细胞内MDA生成量减少.同时,细胞内主要抗氧化酶(CAT、SOD和GSH-px)含量也较对照组增加,并呈剂量效应关系.结果提示,预先经FTE的保护可有效地对抗H2O2诱发的LLC-PK1细胞氧化损伤.

Protective Effect of Fuzhuan Brick Tea on H2O2-Induced Cellular Oxidative Damage in LLC-PK1 Cells

The aim of this study is to investigate the protective effect of ethanolic extract from Fuzhuan brick tea(FTE) on H2O2-induced oxidative damage in LLC-PK1 cells.Various concentration(10～200 μg/mL) of FTE were pre-incubated with LLC-PK1 cells for 24 h,and then exposed to H2O2(500 μmol/L) for 4 h.The cell viability was determined by MTT assay,contents of malondialdehyde(MDA) were determined by thiobarbituric acid reactive substances(TBARS) assay.Intracellular antioxidant enzyme activities,including catalase(CAT),superoxide dismutase(SOD) and glutathione peroxidase(GSH-px) were determined by colorimetric assay according to the protocols of the commercial kit.The cell viabilities of H2O2 treated LLC-PK1 cells were increased,and the contents of MDA were decreased after 24 h pre-incubated with different concentrations of FTE.In addition,the antioxidant enzymes(CAT,SOD and GSH-px) levels were also increased in FTE compared with H2O2 treated group.There was statistical significant difference between the FTE treated groups and untreated H2O2 treated groups(all P<0.05).Those results suggested that pre-incubated with FTE showed a protective effect against H2O2-induced oxidative damage in LLC-PK1 cells.