利用ISSR标记分析36份番石榴种质资源的亲缘关系

采用ISSR分子标记技术对36份番石榴种质资源的亲缘关系进行分析,PCR产物经聚丙烯酰胺凝胶电泳后,通过非加权配对算术平均法(UPGMA)进行数据分析,建立种质资源间的亲缘关系;从100条ISSR引物中筛选出9条多态性较丰富且稳定的引物,共扩增出165条带,其中多态性条带129条,多态性百分率为78.18%;扩增结果可将36份种质资源较好地区分开,各种质间遗传相似系数为0.70~0.93,平均遗传相似系数为0.815;在遗传相似系数0.75处,可将36份种质资源分成4组,与形态分类结果大致相符合。结果表明所收集的番石榴种质资源遗传基础较狭窄,但ISSR分子标记技术能较好地鉴别这些种质资源。

Genetic Relationship among Guava Germplasms Accessed by Using ISSR Markers

Using ISSR molecular markers,PCR products of 36 guava germplasms were purified by polyacrylamide gel electrophoresis and photographed the images for a UPGMA analysis to establish the genetic relationship.Out of 100 UBC series ISSR primers, 9 rich and stable polymorphic primers were selected and 165 bands amplified.Among the 165 bands, 129 were polymorphic rendering a polymorphism percentage of 78.18%.The amplified results could satisfactorily distinguish the 36 germplasms.The genetic similarity coefficient of all samples ranged from 0.70 to 0.93,averaging 0.815.At the coefficient of 0.75, the 36 germplasms were divided into 4 groups.The results were consistent with the morphological classification.Although the genetic basis for classification was weak,with the aid of ISSR molecular markers,clear identifications for the individual germplasms of the collection were obtained.