| 小菜蛾储存蛋白基因的克隆与表达分析 |
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| 引用本文： | 陈伟军,尤燕春,仲帆,任娜娜,许贤美,谢苗. 小菜蛾储存蛋白基因的克隆与表达分析[J]. 福建农林大学学报(自然科学版), 2017, 46(3). DOI: 10.13323/j.cnki.j.fafu(nat.sci.).2017.03.013 |
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| 作者姓名： | 陈伟军 尤燕春 仲帆 任娜娜 许贤美 谢苗 |
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| 作者单位： | 1. 福建农林大学应用生态研究所;福建农林大学生命科学学院;农业部闽台作物有害生物综合治理重点实验室;闽台特色作物病虫生态防控协同创新中心,福建福州350002;2. 福建农林大学生命科学学院;3. 福建农林大学应用生态研究所;农业部闽台作物有害生物综合治理重点实验室;闽台特色作物病虫生态防控协同创新中心,福建福州350002 |
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| 摘 要： | 克隆和分析了2个小菜蛾的储存蛋白基因——Px AJSP-1和Px BJHSP-2.其中,Px AJSP-1属于芳基贮存蛋白,而Px BJHSP-2属于富甲硫氨酸储存蛋白.进化树分析表明,它们在同一目昆虫中相对保守.利用实时荧光定量PCR分析其表达模式,发现Px AJSP-1和Px BJHSP-2在4龄幼虫和蛹期高表达,且在脂肪体中的表达量远高于其他组织.
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| 关 键 词： | 储存蛋白 小菜蛾 实时荧光定量PCR 表达分析 |
Characterization and expression profiling of storage protein genes in Plutella xylostella ( L.) |
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| Abstract: | Two storage protein genes in Plutella xylostella, PxAJSP-1 and PxBJHSP-2, were cloned and characterized. The results showed that PxAJSP-1 belongs to the arylphorin-like protein and PxBJHSP-2 belongs to the methionine-rich protein. Phylogenetic tree analyses revealed that both genes were relatively conservative among the insects of the same order. The expression pattern analy-ses by quantitative real-time PCR ( qPCR) showed that PxAJSP-1 and PxBJHSP-2 were highly expressed in the fourth-instar larvae and pupae, respectively. And their expressions predominated in fat body than other tissues. |
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| Keywords: | hexamerins Plutella xylostella qPCR expression profile |
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