| 产鸭嘴花酚碱芽胞杆菌的筛选 |
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| 引用本文： | 郑梅霞,陈峥,刘波,朱育菁,潘志针,史怀.产鸭嘴花酚碱芽胞杆菌的筛选[J].福建农业学报,2017,32(1). |
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| 作者姓名： | 郑梅霞 陈峥 刘波 朱育菁 潘志针 史怀 |
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| 作者单位： | 福建省农业科学院农业生物资源研究所,福建福州,350003 |
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| 基金项目： | 国家自然科学基金，福建省重大专项项目农业科技重大专项，福建省科技计划项目——省属公益类科研院所基本科研专项 |
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| 摘 要： | 采用液相-四级杆飞行时间质谱的方法结合Metlin数据库检索分析14株芽胞杆菌菌株发酵液的胞外成分。筛选出一株产鸭嘴花酚碱的芽胞杆菌菌株为甲基营养型芽胞杆菌Bacillus methylotrophicus FJAT-16022,其中该成分含量占其发酵液总代谢物含量的1.39%,匹配得分为95.1。表明甲基营养型芽胞杆菌芽胞杆菌Bacillus methylotrophicus FJAT-16022为鸭嘴花酚碱高产菌株,该研究为芽胞杆菌来源鸭嘴花酚碱活性物质的开发与利用提供理论依据。
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| 关 键 词： | 液相色谱/四极杆飞行时间质谱 甲基营养型芽胞杆菌 鸭嘴花酚碱 |
A Vasicinol-producing Bacillus-like Bacterium |
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| ZHENG Mei-xia,CHEN Zheng,LIU Bo,ZHU Yu-jing,PAN Zhi-zhen,SHI Huai.A Vasicinol-producing Bacillus-like Bacterium[J].Fujian Journal of Agricultural Sciences,2017,32(1). |
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| Authors: | ZHENG Mei-xia CHEN Zheng LIU Bo ZHU Yu-jing PAN Zhi-zhen SHI Huai |
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| Abstract: | A vasicinol-producing strain of Bacillus methylotrophicus FJAT-16022 was screened from 14 Bacillus-like bacteria with an aid of liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOF MS) combined with retrieval comparison against the mass spectrometry database,Metlin.The obtained vasicinol had a matching score of 95.1 with the database,and its relative content was up to 1.73％ of the total metabolites in the cultural supernatants.It appeared that B.methylotrophicus FJAT-16022 was a high-yield strain in producing vasicinol.The result obtained paved the way for the development and utilization of vasicinol from the identified bacterium. |
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| Keywords: | liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry Bacillus methylotrophicus vasicinol |
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