大孔树脂柱吸附纯化双孢蘑菇多糖的工艺

对HZ830和D303两种大孔树脂串联纯化双孢蘑菇多糖的工艺进行了研究,考察体积流速、上样量和洗脱剂用量对双孢蘑菇多糖色素脱除率、蛋白脱除率和多糖保留率的影响。结果表明在以HZ830树脂为前柱,D303树脂为后柱,2种树脂等体积装柱,上样质量浓度为20mg·mL~(-1) (pH 8),上样体积5倍树脂柱床体积(5BV,260mL),体积流速为1.73mL·min~(-1),上样结束后以1倍树脂柱床体积(1BV,52mL)纯水洗脱的工艺条件下,对蘑菇预煮液浓缩液中提取的双孢蘑菇粗多糖的蛋白质和色素杂质的脱除率分别为91.57%和94.14%,多糖保留率为73.49%。

Purification of Polysaccharide Extract from Agaricus bisporusUsing Macroporous Resins

Macroporous resins, HZ830 and D303, were used for the purification of polysaccharide extractfrom Agaricus bisporus.The evaluation criteria for the process included there movalrates of pigment and protein as well as the retention of polysaccharides in the filtrate under varied flow rates, feeding volumes and eluent flushing on the macroporous resin filtration system.The resin columns were assembled in series with either a HZ830 column in front or behind a D303 column.It was found that the best results could be achievedusing a HZ830 column followed by a D303 column andby feeding through themat a flow rate of 1.73 mL·min-1 of 5 times of the bed volume (BV,260 mL) of a 20 mg·mL-1 polysaccharide extractat pH 8.0,the bed volume of colum were 52 mL,then flushing with 1 BV(52 mL) of water at the end.The resulting decolorization, deproteinization and polysaccharide retention rates of the filtration on thehot water mushroom extractreached 94.14%, 91.57% and 73.49%, respectively.