酶法修饰绿茶多糖对免疫低下模型小鼠免疫活性的影响

以糖苷酶溶液为工具酶,研究酶法修饰茶多糖对环磷酰胺抑制的免疫低下模型小鼠的免疫功能的影响。结果表明,原多糖TPS与改性后的茶多糖ETPS在免疫低下模型小鼠的细胞免疫功能、体液免疫功能、单核-巨噬细胞功能和NK细胞活性4个方面的实验中,结果均呈阳性,说明它们均具有明显的增强免疫功能的作用,酶法修饰改性能显著提高绿茶多糖的免疫活性。在同等剂量下,ETPS与TPS相比能提高脾指数、腹腔巨噬细胞吞噬率和吞噬指数,能显著提高DTH试验耳肿程度、血清HC50、NK细胞活性。绿茶多糖通过酶法修饰免疫活性得到增强。

Effect of Enzymatic Modification Green Tea Polysaccharide on Immune Function of the Immunosuppressant Mice

Green tea polysaccharides was modified by using glycosidase extracted from tea,the effect of enzymatic modification of green tea polysaccharides on immune function of the immunosuppressant mice inhibited by cyclophosphamide was studied.The results showed that,TPS and ETPS were positive in the cell immune function,humor immune function,mononuclear macrophage function and NK cell activity tests.So it can be concluded that they could obviously improve both the cell immune function and humor immune function in immunosuppressant mice.The results also made clear that the enzymatic modification could significantly enhance the immune activity of green tea polysaccharides.With the same dose,ETPS could improve the spleen index,the phagocytosis rate and index of peritoneal macrophage,the level of ear swell in DTH,HC50 in serum and NK cell activity compared with the original TPS.Collectively,these data proved that the immune activity of green tea polysaccharides after enzymatic modification was significantly improved.