| 黄金宝树组培育苗技术研究 |
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| 引用本文： | 张月娇.黄金宝树组培育苗技术研究[J].福建林业科技,2012,39(3):115-119. |
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| 作者姓名： | 张月娇 |
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| 作者单位： | 福建省林业科技试验中心,福建南靖,363600 |
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| 基金项目： | 福建省林业厅种业创新与产业化工程项目(编号:2130106技术推广与培训) |
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| 摘 要： | 以黄金宝树基部半木质化萌芽条为外植体,MS为基本培养基,探讨6-BA、KT、NAA对黄金宝树组培育苗的影响,试验结果表明,黄金宝树组培育苗最佳培养基配方分别为:初代培养基MS+6-BA 0.3 mg.L-1+KT 0.5 mg.L-1+NAA 0.3mg.L-1+蔗糖30 g.L-1+卡拉胶8 g.L-1、增殖培养基MS+6-BA 0.5 mg.L-1+KT 0.3 mg.L-1+NAA 0.3mg.L-1+蔗糖30 g.L-1+卡拉胶8 g.L-1、生根培养基1/2 MS+NAA 0.5 mg.L-1+蔗糖20 g.L-1+卡拉胶8 g.L-1。初代培养诱导率达80%以上,有效芽条增殖率达3倍以上,生根率达95%以上,移栽成活率达90%以上。
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| 关 键 词： | 黄金宝树 组织培养 育苗技术 |
Melaleuca bracteata Tissue Culture and Seedling Raising Technology Research |
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| ZHANG Yue-jiao.Melaleuca bracteata Tissue Culture and Seedling Raising Technology Research[J].Journal of Fujian Forestry Science and Technology,2012,39(3):115-119. |
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| Authors: | ZHANG Yue-jiao |
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| Institution: | ZHANG Yue-jiao(Fujian Forestry Science and Technology Test Center,Nanjing 363600,Fujian,China) |
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| Abstract: | Melaleuca bracteata base semi-lignified buds were took as explants,MS was took as basic medium to explore the 6-BA,KT,NAA effects on Melaleuca bracteata tissue culture and seedling raising,he results showed that the best Melaleuca bracteata tculture medium was as follows: initial medium MS+6-BA0.3 mg·L-1+KT0.5 mg·L-1+NAA0.3 mg·L-1+ sucrose 30 g·L-1+the carrageenan 8 g·L-1,the multiplication medium MS+6-BA0.5 mg·L-1+KT0.3 mg·L-1+ NAA0.3 mg·L-1+ sucrose 30 g·L-1+carrageenan 8 g·L-1,the rooting medium 1/2MS+NAA0.5 mg·L-1+sucrose 20 g·L-1+carrageenan 8 g·L-1,transplanting light matrix was 50% of peat soil+50% of sawdust.Primary culture induced rate was more than 80%,effective bud proliferation rate was more than 3 times,the rooting rate was more than 95%,survival rate of transplant was more than 90%. |
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| Keywords: | Melaleuca bracteata tissue culture nursery technology |
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