给猪注射pGRF基因质粒安全性的研究

将56头35日龄断奶DLY仔猪,随机分为4个处理,每个处理2个重复,每个重复7头猪。4个处理分别为对照组、3 mg组(第1天注射3 mg pGRF)、6 mg组(第1、45天分别注射3 mg pGRF)、9 mg组(第1天注射3 mgGRF、第45天注射6 mg pGRF)。到150 d时结束饲养试验,每个处理选择8头猪进行放血屠宰。屠宰前采试猪的全血10 mL制备血浆,分别测定血浆GH、SS和GRF的含量;屠宰后取心、肝、脾、肺和肾各一块用于制作组织切片;另取心、肝、脾、肺、肾、注射质粒部位肌肉和非注射部位肌肉各一块用于检测质粒的残留。结果表明:屠宰前各处理血浆激素的浓度没有显著差异(P>0.05),注射3 mg和6 mg使猪肝脏的器官系数显著下降(P<0.05),对其他器官和功能没有产生不良影响;注射9 mg使肝脏和肾脏产生广泛的颗粒变性。质粒的残留仅在9 mg组注射部位肌肉中能检测到。综合各项试验结果6 mg以下注射剂量的pGRF基因质粒在养猪生产上的使用是安全的。

Study on the Security of pGRF Used in Pigs

56 three-line crossbred piglets weaned at 35 days old were allotted to 4 treatments,with(initial weight7.82±0.26) kg,with 2 replicates in per treatment.The treatments were:(1) Saline was injected;(2) 3 mg pGRF was injected at the first day of the experiment;(3) 3 mg pGRF was injected at the first day,3 mg was injected again 45 days later;(4) 3 mg pGRF was injected at the first day,6mg was injected again 45 days later.Feeding experiment was ended at the 150 th day,8 pigs in per treatment were slaughtered.After slaughter a bit of heart,liver,spleen,lung and kidney were taken from each pig for histopathology observation.A bit of heart,liver,spleen,lung,kidney,muscle from injected position and non-injected position was taken to detect the remnant of plasmid.Results showed injection of 3 mg and 6 mg pGRF significantly decreased liver's weight of pigs,but hadn't any harm to pig's health in other aspects.Injection of 9 mg made granule degeneration happen in liver and kidney.The plasmid's remnant could only been detected in the muscle of injected position in 9 mg group.All of these suggested the injection dosage don't exceed 6 mg was safe for pig.