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| 紫花苜蓿MsFPS基因超表达载体的构建及烟草转化 | |
| 引用本文： | 赵惠娟,杨青川,金洪,康俊梅,孙彦,龙瑞才.紫花苜蓿MsFPS基因超表达载体的构建及烟草转化[J].中国草地学报,2011,33(6). |
| 作者姓名： | 赵惠娟 杨青川 金洪 康俊梅 孙彦 龙瑞才 |
| 作者单位： | 1. 内蒙古农业大学农学院,内蒙古 呼和浩特010010;中国农业科学院北京畜牧兽医研究所,北京100193 2. 中国农业科学院北京畜牧兽医研究所,北京,100193 3. 内蒙古农业大学农学院,内蒙古 呼和浩特,010010 4. 中国农业大学动物科技学院,北京,100193 5. 重庆大学农学与生命科学学院,重庆,400030 |
| 基金项目： | 现代农业产业技术体系建设专项资金 |
| 摘 要： | 采用RT-PCR技术获得紫花苜蓿的法呢基焦磷酸合成酶基因,并连接到含有35S启动子和GUS基因的载体pBI121上,成功构建植物表达载体pBI-FPS.用根癌农杆菌介导法将其转入烟草中,共获得7株卡那霉素抗性苗,对其中的4株进行PCR检测,证明目的基因已经整合到烟草基因组中.进一步对这4株进行RT- PCR和组织化学染色法检测,初步证实目的基因在烟草中可以表达,说明已成功获得能够表达MsFPS基因的转基因烟草. |
| 关 键 词： | 紫花苜蓿 MsFPS基因 表达载体构建 烟草转化 |
Construction of Super-expression Plasmid of MsFPS Gene from Medicago sativa L.and Transformation of Tobacco |
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| ZHAO Hui-juan,YANG Qing-chuan,JIN Hong,KANG Jun-mei,SUN Yan,LONG Rui-cai.Construction of Super-expression Plasmid of MsFPS Gene from Medicago sativa L.and Transformation of Tobacco[J].Chinese Journal of Grassland,2011,33(6). | |
| Authors: | ZHAO Hui-juan YANG Qing-chuan JIN Hong KANG Jun-mei SUN Yan LONG Rui-cai |
| Institution: | ZHAO Hui-juan1,2,YANG Qing-chuan2,JIN Hong1,KANG Jun-mei2,SUN Yan3,LONG Rui-cai4(1.College of Agronomy,Inner Mongolia Agricultural University,Hohhot 010019,China,2.Institute of Animal Sciences,CAAS,Beijing 100193,3.College of Animal Science and Technology,China Agricultural University,Beijing 010019,China.4 Institute of Agricultural and Life Science,Chongqing University,Chongqing 400044,China) |
| Abstract: | By RT-PCR technology,the cDNA of FPS(Farnesyl phosphate synthase) gene of alfalfa(Medicago sativa L.) was obtained,and it was inserted into plasmid pBI121 that contains promoter 35S and GUS gene.A plant super expressed vector of MsFPS gene from alfalfa was constructed successfully and was transferred to tobacco by the Agrobacterium mediated transformation system.Seven resistant plants to kanamycin were obtained.PCR analysis of four of them showed that the target gene had been integrated into the genomes of ... |
| Keywords: | Medicago sativa MsFPS gene Construction of expression vector Tobacco transformation |
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