| 米槠叶片基因组DNA的提取及分析 |
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| 引用本文： | 林莉莉,郭丽倩,陈潇潇,游章湉,游水生,曹世江.米槠叶片基因组DNA的提取及分析[J].福建林业科技,2019(2):25-29. |
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| 作者姓名： | 林莉莉 郭丽倩 陈潇潇 游章湉 游水生 曹世江 |
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| 作者单位： | 福建农林大学林学院;浙江大学农业与生物技术学院;中国科学院华南植物园退化生态系统植被恢复与管理重点实验室中国科学院华南植物园广东省应用植物学重点实验室 |
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| 基金项目： | 福建省林业推广项目(K8517202A);福建农林大学A类毕业生科研启动费(KXMLT001A) |
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| 摘 要： | 以米槠(Castanopsis carlesii)群落的叶片为材料,采用改良的CTAB法提取米槠基因组DNA,并对获得的基因组DNA进行浓度检测、PCR扩增、琼脂糖凝胶电泳检测、含量测定分析以及扩增结果测序。结果表明:米槠叶片提取的基因组DNA质量和浓度较好,具有良好的完整性,PCR效果好,电泳图谱条带显示清晰,测序结果长度符合要求。利用该方法提取的DNA可用于米槠遗传多样性的分析,可进一步为米槠和格氏栲等其它栲属植物的DNA提取以及其它分子研究提供参考。
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| 关 键 词： | 米槠 DNA提取 CTAB PCR |
Extraction and Analysis of Genomic DNA from Leaves of Castanopsis carlesii |
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| LIN Lili,GUO Liqian,CHEN Xiaoxiao,YOU Zhangtian,YOU Shuisheng,CAO Shijiang.Extraction and Analysis of Genomic DNA from Leaves of Castanopsis carlesii[J].Journal of Fujian Forestry Science and Technology,2019(2):25-29. |
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| Authors: | LIN Lili GUO Liqian CHEN Xiaoxiao YOU Zhangtian YOU Shuisheng CAO Shijiang |
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| Institution: | (College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China;College of Agriculture & Biotechenology, Zhejiang University, Hangzhou 310027, Zhejiang, China;Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems in South China Botanical Garden, Chinese Academy of Sciences, Key Laboratory of Applied Botany of Guangdong Province, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, Guangdong, China) |
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| Abstract: | LIN Lili;GUO Liqian;CHEN Xiaoxiao;YOU Zhangtian;YOU Shuisheng;CAO Shijiang(College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China;College of Agriculture & Biotechenology, Zhejiang University, Hangzhou 310027, Zhejiang, China;Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems in South China Botanical Garden, Chinese Academy of Sciences, Key Laboratory of Applied Botany of Guangdong Province, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, Guangdong, China) |
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| Keywords: | Castanopsis carlesii DNA extraction CTAB PCR |
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