Leek Proliferation: A New Phytoplasma Disease in the Czech Republic and Italy |
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Authors: | A Bertaccini J Fránová S Paltrinieri M Martini M Navrátil C Lugaresi J Nebesárová M Simkova |
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Institution: | (1) Istituto di Patologia Vegetale, University of Bologna, via F. Re 8, 40126 Bologna, Italy; (E-mail;(2) Department of Plant Virology, IPMB, ASCR, Branisovska 31, 37005 eské Budjovice, Czech Republic;(3) Department of Cell Biology and Genetics, Faculty of Science, Palacky University, Slechtitelu 11, 78371 Olomouc – Holice, Czech Republic;(4) Servizio fitosanitario regionale, Cesena, Forli, Italy;(5) Laboratory of Electron Microscopy, Institute of Parasitology, ASCR, Branisovská 31, 37005 eské Budjovice, Czech Republic |
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Abstract: | During the summer 1996, twelve of twenty-eight leek plants located in a garden near eské Budjovice, South Bohemia exhibited symptoms typical of diseases associated with phytoplasmas. In summer 1998 similar symptoms were detected in leek plants in a field used for seed production located in Romagna, North Italy. In both cases the plants were established in the spring of the previous year. Plants showed flower abnormalities: stamen elongation, anther sterility, pistil proliferation, as well as poor, if any, seed production. Phytoplasma-like structures were detected by scanning and transmission electron microscopy in phloem sieve elements in the Czech diseased plants, but not in healthy ones. Nested-PCR amplifications of extracted DNA with phytoplasma-specific oligonucleotide primer pairs confirmed the presence of phytoplasmas in these plants at low concentrations. Restriction fragment length polymorphism analyses of amplified ribosomal sequences allowed the identification of detected phytoplasmas: all the samples from the Czech Republic contained aster yellows related phytoplasmas (16SrI-B) while in the Italian samples aster yellows related phytoplasmas (16SrI-B) together with stolbur related phytoplasmas (16SrXII-A) were identified. This is the first report of detection and identification of a phytoplasma disease of leek in the Czech Republic and Italy. |
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Keywords: | electron microscopy molecular identification PCR RFLP |
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