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酶水解乳清蛋白工艺条件的研究
引用本文:张俊英,王彩云,靳烨,云战友.酶水解乳清蛋白工艺条件的研究[J].农产品加工.学刊,2009(6).
作者姓名:张俊英  王彩云  靳烨  云战友
作者单位:1. 内蒙古农业大学,食品科学与工程学院,内蒙古,呼和浩特,010018
2. 内蒙古伊利实业集团股份有限公司技术中心,内蒙古,呼和浩特,010080
摘    要:采用4因素二次正交旋转实验,对蛋白酶A水解乳清蛋白的工艺条件(pH值、温度、酶与底物浓度比、水解时间)进行优化。建立了以乳清蛋白水解度为响应值的二次正交旋转回归模型,依据该模型确定以乳清蛋白水解度为响应值的最佳水解条件分别为:初始pH值为6.87,温度为59.6℃,酶与底物浓度比为2000.31 U/g,水解时间为2.91 h。

关 键 词:乳清蛋白  水解度  蛋白酶A  优化  模型

Study on the Technology for Hydrolysis of Whey Protein
Zhang Junying,Wang Caiyun,Jin Ye,Yun Zhanyou.Study on the Technology for Hydrolysis of Whey Protein[J].Nongchanpin Jlagong.Xuekan,2009(6).
Authors:Zhang Junying  Wang Caiyun  Jin Ye  Yun Zhanyou
Institution:1.College of Food Science and Engineering;Inner Mongolia Agricultural University;Hohhot;Inner Mongolia 010018;China;2.Technology Center;Inner Mongolia Yili Industrial Group Co.;Ltd;Inner Mongolia 010080;China
Abstract:The conditions of hydrolyzing whey protein by Protease A were studied and the regression model of four factors twice rotation perpendicular regressive design was adopted to optimize the parameters which included pH,temperature,enzyme to substrate ratio(E∶S) and time of hydrolysis.The model on the degree of hydrolysis of whey protein was established and the optimal hydrolysis conditions were as follows:pH in the beginning=6.87,temperature=59.6 ℃,E∶S=2 000.31 U/g,time of hydrolysis=2.91 h.
Keywords:whey protein  degree of hydrolysis  protease A  optimization  model  
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