上海地区新发猪流行性腹泻病毒的鉴定及分析

目前,仔猪腹泻病严重危害我国生猪养殖业,利用核酸分析技术我们对今春导致上海地区多个猪场仔猪腹泻病的病原体进行了鉴定,结果显示,猪腹泻病病毒(PEDV)是致病元凶.在此基础上,我们利用PEDV特异性引物,克隆了上海地区流行毒株的结构蛋白基因S、N和M,并根据保护性抗原S1基因序列比对,分析了此病毒株与其他相关毒株的亲缘关系.结果表明,上海地区PEDV流行株与2010年来我国各地发生的多数猪腹泻病毒亲缘关系接近,而与传统病毒株如CV777和周边日本和韩国近年来流行的毒株存在一定的差异.另一方面,分析还表明,近年来我国不同地区流行的PEDV存在着异质性,亲缘关系存在差异.通过这些分析,提示当前急需研制新的疫苗尤其是减毒活疫苗对PEDV予以预防.

Identification and Analysis of Porcine Epidemic Diarrhea Virus in Shanghai

Up to now,pig husbandry is still seriously threatened by porcine epidemic diarrhea in China.With nucleic acid technologies,porcine epidemic diarrhea virus (PEDV) was identified as the pathogen which caused serious epidemic diarrhea in piglets in Shanghai during spring of 2012.Structural protein genes including S,N and M of the isolate were amplified and cloned.The phylogenetic relationships among the Shanghai isolate and other reference strains were analyzed based on S1 gene which is the main protective protein gene of PEDV.It indicated that the Shanghai isolate shared great similarities with most other isolates reported in China from 2010.However there still existed heterogeneities with isolates from classic strains including CV777 prevalent strains in Japan,South Korea and some areas of China presently.As the result,effective PEDV vaccine is needed for preventing endemic disease of PEDV in piglets.