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球孢白僵菌不同分离株生物学特性及对茶丽纹象甲成虫的杀虫活性研究
引用本文:王定锋,曾明森,王庆森,刘丰静,吴光远.球孢白僵菌不同分离株生物学特性及对茶丽纹象甲成虫的杀虫活性研究[J].福建农业学报,2012,27(7):750-754.
作者姓名:王定锋  曾明森  王庆森  刘丰静  吴光远
作者单位:福建省农业科学院茶叶研究所,福建 福安,355015
基金项目:国家茶叶产业技术体系项目,国家国际科技合作项目,福建省现代农业(茶叶)产业技术体系建设项目,福建省财政专项——福建省农业科学院创新团队建设项目
摘    要:测定了从球孢白僵菌侵染的茶丽纹象甲虫尸上分离的8个分离株的生长速率、产孢量和孢子萌发率等生物学性状,以及对茶丽纹象甲成虫的杀虫活性,旨在筛选出感染该虫的高致病力菌株.结果表明:在萨氏培养基(SDAY)上,8个分离株从菌落形态上可分为菌丝型和孢子型两类.其中,菌丝型菌株XJBb3001和XJBb3007生长速率最快,但菌...

关 键 词:球孢白僵菌  茶丽纹象甲  分离株  生物学  杀虫活性

Biological Characteristics and Insecticidal Activities of Different Beauveria bassiana Isolates Against Myllocerinus aurolineatus
WANG Ding-feng , ZENG Ming-sen , WANG Qing-sen , LIU Feng-jing , WU Guang-yuan.Biological Characteristics and Insecticidal Activities of Different Beauveria bassiana Isolates Against Myllocerinus aurolineatus[J].Fujian Journal of Agricultural Sciences,2012,27(7):750-754.
Authors:WANG Ding-feng  ZENG Ming-sen  WANG Qing-sen  LIU Feng-jing  WU Guang-yuan
Institution:(Tea Research Institute,Fujian Academy of Agricultural Sciences,Fu′an,Fujian 355015,China)
Abstract:In order to rescreen high virulence strains against Myllocerinus aurolineatus,the growth rate,sporulation,germination rate and insecticidal activity of eight Beauveria bassiana isolates,which were isloated from dead body of M.aurolineatus,were investigated.The results showed that the eight isolates could be seperated into two groups: hyphae type and spore type on the SDAY medium.The hyphae type strains of XJBb3001 and XJBb3007 grew faster than the others,but they are useless hyphae and no producing spores.The strain XJBb3005 had the largest number of sporulation,and there were very significant differences in sporulation number between strain XJBb3005 and other isolates.There is no difference among the spore germination rate of the strain XJBb3002,XJBb3003,XJBb3004,XJBb3005,XJBb3006,XJBb3008.Bioassay evaluation were carried out by dipping in spore suspension(1.0×107 spores·mL-1),and the results showed that the isolate XJBb3005 had highest virulent,and corrected mortality of M.aurolineatus reached 100% at 7 d after treatment.The LT50 value was 3.46 d,LT90 value was 5.45 d,and infected rate was 93.75%,respectively.
Keywords:Beauveria bassiana  Myllocerinus aurolineatus Voss  isolates  biology  insecticidal activity
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