| MLST分型技术在链球菌属中的相关参数 |
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| 引用本文： | 张颖,王卓林,张志美,王艳萍,徐倩倩,郭时金,沈志强.MLST分型技术在链球菌属中的相关参数[J].家畜生态,2013(12):76-81. |
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| 作者姓名： | 张颖 王卓林 张志美 王艳萍 徐倩倩 郭时金 沈志强 |
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| 作者单位： | [1]山东省滨州畜牧兽医研究院,山东滨州256600 [2]山东绿都安特动物药业有限公司,山东滨州256600 [3]哈尔滨北方森林动物园,黑龙江哈尔滨150322 |
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| 摘 要： | MLST分型技术是一种基于核酸序列测定的基因分型方法，该方法对多个管家基因进行序列分析，通过不同管家基因的分型来确定最终的序列分型。论文介绍了MLST技术在无乳链球菌、停乳链球菌似马亚种、肺炎链球菌、化脓性链球菌和猪链球菌中的管家基因数目，引物序列和扩增片段大小，以期为微生物的分型奠定基础。
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| 关 键 词： | MLST 链球菌 相关参数 管家基因 |
The Correlation Coefficient of MLST in Streptococcus |
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| ZHANG Ying,WANG Zhuo-lin,ZHANG Zhi-mei,WANG Yan-ping,XU Qian-qian,GUO Shi-jin,SHEN Zhi-qiang.The Correlation Coefficient of MLST in Streptococcus[J].Ecology of Domestic Animal,2013(12):76-81. |
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| Authors: | ZHANG Ying WANG Zhuo-lin ZHANG Zhi-mei WANG Yan-ping XU Qian-qian GUO Shi-jin SHEN Zhi-qiang |
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| Institution: | 1. Binzhou Animal Science ＆ Veterinary Medicine Institute, Binzhou, Shandong 256600, China ; 2. Shandong Lvdu Ante Animal Pharmaceutical Co. Ltd. , Binzhou, Shandong 256600, China 3. Harbin Northern Forest Zoo, Harbin, Heilongjiang 150322,China) |
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| Abstract: | Multi-locus sequence typing （MLST）is a newly defined method that is used for determining the sequences of nucleic acid. It is used to analyze the sequences of several house-keeping genes and help fix the final sequence types. The review was aimed to summarize the advantage and the application of MLST in Streptococus agalactiae, Streptococcus dysgalactiae subspecies equisimilis, Streptococcus pneu- monia, Streptococcus pyogenes and Streptococcus suis, including the amount of housekeeping genes, the primer sequences and the size of the PCR products. |
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| Keywords: | multi-locus sequence typing streptococcus correlation coefficient housekeeping gene |
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