| 转基因兔胚胎玻璃化冷冻保存的研究 |
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| 引用本文： | 陈学进,曾申明.转基因兔胚胎玻璃化冷冻保存的研究[J].中国养兔杂志,1999(2):16-20. |
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| 作者姓名： | 陈学进 曾申明 |
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| 作者单位： | 中国农业大学动物科技学院 |
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| 摘 要： | 在２５℃条件下，将兔体外受精精子载体转基因兔桑椹胚置于含有４０％乙二醇、１８％Ｆｉｃｏｌ、０．３ｍｏｌ蔗糖的ｍＰＢＳ溶液（ＥＦＳ４０）中平衡２分钟，然后直接投入液氮，成功地进行了玻璃化冷冻保存。解冻后桑椹胚发育至囊胚和孵化囊胚的比例分别为６５．８１％和３９．２４％，与未经冷冻的鲜胚发育比例（７１．０５％和４３．４２％）相比，没有明显的差异。７８枚经玻璃化冷冻和解冻的桑椹胚移植给５只受体，其中２只妊娠，共产下８只活仔兔。
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| 关 键 词： | 转基因 兔 胚胎 玻璃化 冷冻保存 |
Studies on the Cryopreservation of Transgenic Rabbit Embryos by Vitrification |
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| Chen,XuejinZeng ShenmingZhu Shien et al..Studies on the Cryopreservation of Transgenic Rabbit Embryos by Vitrification[J].Chinese Journal of Rabbit Farming(CJRF),1999(2):16-20. |
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| Authors: | Chen XuejinZeng ShenmingZhu Shien |
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| Abstract: | Rabbit morulae produced from IVF transgenic
embryos by sperm - mediated were successfully cryopreserved by vitrification with mPBS
containing 40% ethlene glycol,18%Ficoll and 0.3 mol sucrose(EFS40).Morulae exposied to
EFS40 were vitrified in liquid nitrogen for 2 minutes at 25.The percentages of the frozen/thawed
morulae developed to blastocysts and hatching blastocysts in vitro were 65.81% and 39.24%
respectively,which were not signifcantly different(P>0.05)from that of the frensh morulae derived
from IVF embryos(71.05% and 41.79%).78 vitrified and warmed morulae were transferred to five
recipients.Two of them became pregnant and eight live young were delivered. |
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| Keywords: | Transgenic Rabbit Embryo Vitrification Cryopreservation |
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