| 我国南方常见的6种寡毛实蝇PCR-RFLP快速鉴定研究 |
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| 引用本文： | 吴佳教,梁帆,胡学难,赵菊鹏,梁广勤. 我国南方常见的6种寡毛实蝇PCR-RFLP快速鉴定研究[J]. 江西农业大学学报, 2004, 26(5): 770-773 |
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| 作者姓名： | 吴佳教 梁帆 胡学难 赵菊鹏 梁广勤 |
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| 作者单位： | 广州出入境检验检疫局,广东,广州,510623;广州出入境检验检疫局,广东,广州,510623;广州出入境检验检疫局,广东,广州,510623;广州出入境检验检疫局,广东,广州,510623;广州出入境检验检疫局,广东,广州,510623 |
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| 基金项目： | 广东省出入境检验检疫局资助项目(GDK14-2001) |
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| 摘 要： | 应用PCR—RFLP技术，对我国南方发生和诱捕到的6种寡毛实蝇开展了快速鉴定方法研究，结果表明，设计出的2组引物对6种供试实蝇线粒体DNA(mtDNA)的PCR扩增片断大小分别约为350bp和450bp。用限制性内切酶MSEI和DRAI对PCR扩增产物进行酶切，得到的酶切位点可将供试的6种实蝇区分开来。该方法不受供试实蝇食物源的影响，对各种虫态(卵、幼虫、蛹)和不同性别的成虫均适用，可用于实蝇的快速鉴定。
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| 关 键 词： | 实蝇 寡毛实蝇 PCR-RFLP 鉴定 |
| 文章编号： | 1000-2286(2004)05-0770-04 |
| 修稿时间： | 2004-07-06 |
A Study on Quick Discrimination Among 6 Species of Fruit Flies(Tephritidae:Bactrocera ) in South China Based on PCR-RFLP |
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| WU Jia-jiao,LIANG FanHU Xue-nan,ZHAO Ju-peng,LIANG Guang-qin. A Study on Quick Discrimination Among 6 Species of Fruit Flies(Tephritidae:Bactrocera ) in South China Based on PCR-RFLP[J]. Acta Agriculturae Universitis Jiangxiensis, 2004, 26(5): 770-773 |
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| Authors: | WU Jia-jiao LIANG FanHU Xue-nan ZHAO Ju-peng LIANG Guang-qin |
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| Abstract: | The method of quick discrimination among 6 species of fruit flies (Tephritidae : Bactrocera) occurred or trapped from the South of China was studied based on PCR-RFLP of the mitochondrial DNA (mtDNA). The results showed that DNAs, which were estimated about 350bp and 450bp respectively, were obtained by means of PCR with two groups of primers designed from mtDNA of target pests. The PCR-products could be registered by restriction Enzymes (MSE I and DRA I). All the 6 species could be discriminated according to the banding patterns of the restriction enzymes. The method was not affected by individuals, which were collected from different host plants. It is suggested that the method is reliable for quick discrimination of fruit flies. |
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| Keywords: | fruit flies Bactrocera PCR-RFLP identification |
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