| 白花紫露草的组织培养与植株再生体系的建立 |
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| 引用本文： | 陈宝鑫,王晓旭,张倩怡,吴军,姜长阳.白花紫露草的组织培养与植株再生体系的建立[J].北方园艺,2009(6). |
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| 作者姓名： | 陈宝鑫 王晓旭 张倩怡 吴军 姜长阳 |
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| 作者单位： | 辽宁师范大学,生命科学学院,辽宁,大连,116029;辽宁师范大学,生命科学学院,辽宁,大连,116029;辽宁师范大学,生命科学学院,辽宁,大连,116029;辽宁师范大学,生命科学学院,辽宁,大连,116029;辽宁师范大学,生命科学学院,辽宁,大连,116029 |
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| 摘 要： | 以白花紫露草嫩茎为材料,进行了愈伤组织诱导、分化、试管苗生根、试管苗移栽所需条件的研究。结果表明：1/2MS＋BA 0.5 mg/L＋NAA 0.2 mg/L是诱导愈伤组织的理想培养基;MS＋BA 1.0 mg/L＋2,4-D 0.5 mg/L是诱导愈伤组织形成,同时具有分化能力愈伤组织的理想培养基;1/2MS＋BA 0.2 mg/L＋NAA 0.1 mg/L是诱导愈伤组织和不定芽分化的理想培养基;1/2MS＋IAA 0.3 mg/L是生根培养的理想培养基;以炉灰渣为试管苗的移栽扦插基质,移栽成活率为98%,扦插成活率为91%。
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| 关 键 词： | 白花紫露草 组织培养 培养基 |
Tissue Culture and Plantlet Regeneration of Tadescantia fluminensis |
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| CHEN Bao-xin,WANG Xiao-xu,ZHANG Qian-yi,WU Jun,JIANG Chang-yang.Tissue Culture and Plantlet Regeneration of Tadescantia fluminensis[J].Northern Horticulture,2009(6). |
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| Authors: | CHEN Bao-xin WANG Xiao-xu ZHANG Qian-yi WU Jun JIANG Chang-yang |
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| Institution: | College of Life Science;Liaoning Normal University;Dalian;Liaoning 116029;China |
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| Abstract: | Tissue culture and plantlet rgeneration of Tadescantia fluminensis with the stem as an explant was studied in order to prove the optimum conditions of callus induction, the differentiation of buds, rooting and transplantation. The results showed that the medium 1/2MS+BA 0.5 mg/L+NAA 0.2 mg/L was the best for inducing callus;MS+BA 1.0 mg/L+2,4-D 0.5 mg/L was the most suitable medium for the callus to differentiate; The opmium medium for forming callus and differentiation of adventitious bud was 1/2MS+BA 0.2 ... |
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| Keywords: | Tradescantia fluminensis Tissue culture Medium |
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