| NaCl胁迫对嫁接西瓜抗氧化酶及叶绿素荧光参数的影响 |
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| 引用本文： | 张爱慧,刘广勤,朱士农,周斌,刘敏.NaCl胁迫对嫁接西瓜抗氧化酶及叶绿素荧光参数的影响[J].江苏农业学报,2010,26(2). |
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| 作者姓名： | 张爱慧 刘广勤 朱士农 周斌 刘敏 |
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| 作者单位： | 1. 金陵科技学院园艺学院,江苏,南京,210038
2. 江苏省农业科学院园艺研究所,江苏,南京,210014 |
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| 基金项目： | 江苏省高校自然科学基础研究项目(07KJD210060);;金陵科技学院科研基金项目(JIT-N-2007008) |
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| 摘 要： | 为研究盐胁迫下嫁接西瓜的耐盐机制,以耐盐性较强的葫芦品种超丰抗生王为砧木,耐盐性较弱的西瓜品种秀丽为接穗,采用营养液水培法,研究了NaCl胁迫对西瓜自根苗和嫁接苗谷胱甘肽抗氧化酶系统、SOD同工酶、POD同工酶及叶绿素荧光参数的影响。结果表明:NaCl胁迫下嫁接苗、自根苗叶片中超氧阴离子和H2O2含量明显增加;嫁接苗叶片和根系中SOD、POD同工酶变化明显不同,不同同工酶活性差异明显。此外,NaCl胁迫下嫁接苗叶片非酶系统抗氧化酶抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)和脱氢抗坏血酸还原酶(DHAR)]活性均显著高于自根苗;嫁接苗叶片中叶绿素荧光参数qP和Fv/Fm降低的幅度小于自根苗。上述结果表明,NaCl胁迫下嫁接苗通过维持较高的抗氧化酶活性以提高活性氧的清除能力,并保持较高的光合效率,从而增强西瓜幼苗对盐胁迫的耐性。
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| 关 键 词： | NaCl胁迫 嫁接西瓜 抗氧化酶 同工酶 叶绿素荧光参数 |
Effects of NaCl Stress on Antioxidant Enzymes and Chlorophyll Fluorescence Parameters in Grafted Watermelon |
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| ZHANG Ai-hui,LIU Guang-qin,ZHU Shi-nong,ZHOU Bin,LIU Min.Effects of NaCl Stress on Antioxidant Enzymes and Chlorophyll Fluorescence Parameters in Grafted Watermelon[J].Jiangsu Journal of Agricultural Sciences,2010,26(2). |
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| Authors: | ZHANG Ai-hui LIU Guang-qin ZHU Shi-nong ZHOU Bin LIU Min |
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| Institution: | 1.College of Horticulture/a>;Institute of Jinling Technology/a>;Nanjing 210038/a>;China/a>;2.Institute of Horticulture/a>;Jiangsu Academy of Agricultural Sciences/a>;Nanjing 210014/a>;China |
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| Abstract: | The changes of protective enzymes activities such as glutathione dependent antioxidant system,SOD isozyme,POD isoenzymes and chlorophyll fluorescence parameters were studied in grafted and own-rooted watermelon seedlings under NaCl stress using calabash cultivar Chaofengkangshengwang as stock and salt intolerant watermelon cultivar Xiuli as scion.The results showed that the contents of superoxide anion and H2O2 were lower in control than those in grafted and own-rooted plants.Isoenzymes of superoxide dismut... |
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| Keywords: | NaCl stress grafted watermelon antioxidant enzyme isozyme chlorophyll fluorescence parameter |
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