| 产氨短杆菌产辅酶A反应体系的优化设计 |
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| 引用本文： | 黄亮,高建英,胡炳旭,訾晨瑞,童应凯.产氨短杆菌产辅酶A反应体系的优化设计[J].天津农学院学报,2008,15(4):44-47. |
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| 作者姓名： | 黄亮 高建英 胡炳旭 訾晨瑞 童应凯 |
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| 作者单位： | 1. 天津农学院农学系,天津,300384
2. 天津市轩宏医药技术有限公司,天津,300384 |
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| 摘 要： | 采用产氨短杆菌体对Coenzyme A（CoA）酶促合成的最优合成条件进行了研究。经过计算机模拟寻优，得到反应液的最优配方为KH2PO4600mg、氯化十六烷吡啶10mg、泛酸钙70mg、MgSO4·7H2O60mg、半胱氨酸盐酸盐130mg，加水定容至100mL（pH6.0）。CoA的最高效价值为29.99×10^3单位/L，比原配方的效价值提高了2.68倍。
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| 关 键 词： | 生物技术 辅酶A 产氨短杆菌 酶促合成 |
Optimization Studies on Culture Medium of Coenzyme A with Brevibacterium ammoniagenes |
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| HUANG Liang,GAO Jian-ying,HU Bing-xu,ZI Chen-rui,TONG Ying-kai.Optimization Studies on Culture Medium of Coenzyme A with Brevibacterium ammoniagenes[J].Journal of Tianjin Agricultural College,2008,15(4):44-47. |
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| Authors: | HUANG Liang GAO Jian-ying HU Bing-xu ZI Chen-rui TONG Ying-kai |
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| Institution: | HUANG Liang~1 GAO Jian-ying~1 HU Bing-xu~1 ZI Chen-rui~2 TONG Ying-kai~1 |
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| Abstract: | The best biosynthesis conditions of the Coenzyme A were studied using Brevibacterium ammoniagenes. Through the computer optimization, the best design is as follows： KH2PO4 600mg, Cetylpyridinium Chloride, Monohydrate （CPC） 10mg,Calcium Pantothenate 70 mg, MgSO4·7H2O60mg, Cysteine Hydrochloride （Anhyarous） 130mg, and water was used to dilute the final volume up to 100 mL （pH 6.0） . The production of Coenzyme A achieved 29.99×10^3, which was 3.68 times the original formula. |
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| Keywords: | biotechology Coenzyme A (CoA) Brevibacterium ammoniagenes enzymatic biosynthesis |
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