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大豆蛋白限制性酶解修饰与产品的溶解性和保水性变化
引用本文:刘宏芳,侯瑶,赵新淮. 大豆蛋白限制性酶解修饰与产品的溶解性和保水性变化[J]. 东北农业大学学报, 2009, 40(1)
作者姓名:刘宏芳  侯瑶  赵新淮
作者单位:1. 哈尔滨市产品质量监督检验院,哈尔滨,150036
2. 乳品科学教育部重点实验室,东北农业大学食品学院,哈尔滨,150030
基金项目:黑龙江省科技厅科技攻关项目 
摘    要:
为改善大豆蛋白的功能性质,利用中性蛋白酶和胰蛋白酶对大豆浓缩蛋白、分离蛋白进行限制性酶解处理,并考察相应产品的酶解修饰模式与溶解性、保水性变化的关系。以酶解修饰蛋白质产品的氮溶解指数、保水率、单分子层水含量为指标,测定水解度(Drgree of Hydrolysis,DH)为1%、2%的8种大豆蛋白酶解修饰产品的溶解性、保水性与水吸附作用。结果表明,限制性酶解修饰处理后,酶解修饰产品的溶解性、保水性、水吸附作用的变化与酶解模式或DH有关;大豆浓缩蛋白经胰蛋白酶修饰至DH为1%,可以显著提高修饰产品的溶解性和保水性;大豆分离蛋白经胰蛋白酶或中性蛋白酶修饰后,可改善溶解性但破坏其保水性;大豆浓缩蛋白、分离蛋白的限制性酶解修饰处理,可以提高修饰产品对水的吸附作用。

关 键 词:大豆浓缩蛋白  大豆分离蛋白  酶水解  溶解性  保水性  水吸附

Limited hydrolysis of soybean proteins and modifications in solubility and water retention capacity of final products
LIU Hongfang,HOU Yao,ZHAO Xinhuai. Limited hydrolysis of soybean proteins and modifications in solubility and water retention capacity of final products[J]. Journal of Northeast Agricultural University, 2009, 40(1)
Authors:LIU Hongfang  HOU Yao  ZHAO Xinhuai
Affiliation:1. Harbin Institute of Product Quality and Inspection;Harbin 150036;China;2. Key Laboratory of Dairy Science;Ministry of Education;Food College;Northeast Agricultural University;Harbin 150030;China
Abstract:
In order to improve functional properties of soybean proteins, two soybean protein products, soybean protein concentrate and soybean protein isolate, were limited hydrolyzed with neutrase or trypsin. The relationship between hydrolysis patterns and modification in solubility, water retention capacity and moisture absorption of hydrolyzed soybean products were studied. Nitrogen soluble index, water retention capacity and water content of monolayer were analyzed for eight hydrolyzed soybean protein products, ...
Keywords:soybean protein concentrate  soybean protein isolate  enzymatic hydrolysis  solubility  water retention capacity  moisture adsorption  
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