| 鲤鱼上皮细胞在不同培养条件下的生长状况及与哺乳动物细胞的比较 |
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| 引用本文： | 崔莹莹,吴东明,李月红,付云红.鲤鱼上皮细胞在不同培养条件下的生长状况及与哺乳动物细胞的比较[J].安徽农业科学,2010,38(24):13138-13139. |
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| 作者姓名： | 崔莹莹 吴东明 李月红 付云红 |
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| 作者单位： | 吉林农业大学,吉林长春,130118 |
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| 基金项目： | 国家自然科学基金项目，吉林省科技厅科技发展计划项目 |
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| 摘 要： | 目的]比较鲤鱼上皮细胞在不同培养条件下的生长状况。方法]对不同培养基、温度、胰蛋白酶浓度、CO2培养条件下鲤鱼上皮细胞（EPC）的生长状况进行观察并进行指标测定。结果]EPC细胞在培养基M199、温度20～25℃、胰蛋白酶浓度0.05%～0.10%条件下生长较好,对CO2的需求不大。结论]该研究结果可为鲤鱼上皮细胞最优培养条件的筛选提供依据。
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| 关 键 词： | 鲤鱼上皮细胞 培养条件 水生动物细胞 |
Comparison of Growth Condition of Epithelioma Papulosum Cyprini in Different Culture Environments and Culture Environment with Cell of Mammals |
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| Institution: | CUI Ying-ying et al (Jilin Agriculture University,Changchun,Jilin 130118) |
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| Abstract: | Objective]The research aimed to compare the growth condition of epithelioma papulosum cyprini in different culture environments.Method]The growth conditions and growth indices of EPC under different medium,temperature,content of trypsase and content of CO2 were determined.Result]The results showed that EPC growed well with medium M199,temperature of 20-25 ℃,trypsase content of 0.05%-0.10%.And it had less need of CO2.Conclusion]This research could provide basis for the screening of the best culture environment of EPC. |
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| Keywords: | Epithelioma papulosum cyprini （ EPC ） Culture environment Cell of aquatic animal |
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