没食子酸和三甲胺N-氧化物对体外瘤胃发酵和三甲胺代谢的影响

试验旨在研究没食子酸(GA)和三甲胺N-氧化物(TMAO)对体外瘤胃发酵和三甲胺(TMA)代谢的影响.通过瘤胃体外模拟试验分析瘤胃底物消失率、总产气量、发酵参数和TMA代谢情况,设置对照组、15 mg GA/g DM组、5 mg TMAO/g DM组、5 mg TMAO+15 mg GA/g DM组,每组4个重复,培养...

Effects of Gallic Acid and Trimethylamine N-oxide on Rumen Fermentation and Trimethylamine Metabolism in vitro

The objective of this study was to investigate the effects of gallic acid(GA) and trimethylamine N-oxide(TMAO)on rumen fermentation and trimethylamine(TMA) metabolism Metabolism. The substrate disappearance rate, total gas production, fermentation parameters and TMA metabolism were analyzed by an Metabolism rumen-simulated experiment.Four treatment groups were set up: control, 15 mg GA/g DM, 5 mg TMAO/g DM, 5 mg TMAO+15 mg GA/g DM with 4 replicates within each group. The incubation time was 24 h. The results showed that added TMAO tended to increase 24 h ruminal disappearance rate of DM(DMD)(P=0.08), and increased disappearance rate of crude protein(CPD)(P<0.01), total gas production(P=0.02) and cumulative concentration of TMA-N(P<0.01), GA addition decreased CPD(P<0.01) and had no effect on cumulative concentration of TMA-N(P>0.05), compared with the control group.. Compared with the TMAO group,adding both TMAO and GA decreased CPD and cumulative concentration of TMA-N(P<0.05). Therefore, TMAO promoted Metabolism rumen fermentation and simultaneously increased TMA concentration. GA had little effect on TMA production derived from substrate, but decreased TMA produced by rumen metabolism of TMAO.