辣椒抗根结线虫相关WRKY基因的分离

 为了确定抗性基因Me3介导表达的抗根结线虫相关WRKY基因的种类及其表达特点，以含Me3基因的辣椒HDA149品种为材料，接种南方根结线虫二龄幼虫，通过同源克隆法获得辣椒4个WRKY新基因片段。利用RT-PCR技术克隆了WRKY-a、WRKY-b、WRKY-c、CaWRKY1、WRKY1和CaWRKY2等 6个WRKY基因的全长cDNA。研究表明，WRKY-a、CaWRKY2基因是受根结线虫诱导表达的；CaWRKY1基因的表达具有组织特异性，在根、叶中表达，在茎中不表达。聚类分析表明WRKY-a、WRKY-c、CaWRKY2蛋白属于第Ⅰ组WRKY蛋白，WRKY-b、CaWRKY1属于第Ⅱc亚组，WRKY1属于第Ⅱa亚组。

Isolation of WRKY Genes in the Incompatible Interaction Between Meloidogyne incognita and Capsicum annuum

Pepper cultivar HDA149 (Capsicum annuum L.) is resistant to Meloidogyne incognita, which is controlled by a single dominant gene Me3. To determine the type of WRKY genes and their expression characteristics during the incompatible interaction between HDA149 and Meloidogyne incognita, homology-based cloning and RT-PCR approaches were used in this study. Four new hot pepper WRKY genes were isolated from HDA149 after challenging inoculation with J2 root-knot nematode and the complete cDNAs of six WRKY genes i.e. WRKY-a, WRKY-b, WRKY-c, CaWRKY1, WRKY1 and CaWRKY2 were amplified. It was shown that the expression of WRKY-a and CaWRKY2 was induced by root-knot nematode. The CaWRKY1 gene expressed differently in various tissues of the plant, e.g. it expressed in roots and leaves but not in stems. Cluster analysis indicated WRKY-a, WRKY-c and CaWRKY2 proteins were clustered into the groupⅠ of WRKY protein, WRKY-b and CaWRKY1 proteins were classified as subgroup Ⅱc, whereas WRKY1 protein in subgroup Ⅱa. WRKY genes regulate HDA149 resistance to root knot nematode together.