新型向日葵细胞质雄性不育系和恢复系的筛选

采用复合油葵杂交种LD06 F1选株自交，在其F2中，有粉株和不育株成对杂交后，用菊芋（H.petiolaris）的全DNA加渗透液的混合液按时涂抹已授粉的葵盘柱头。经多代分离选育，育成了不育系CMSUA5-1和相应的保持系UB5-2。CMSUA5-1与现有不育系CMSPET-1的恢复系杂交多代试验中，不能被CMSPET-1的恢复系所恢复，证明现有的恢复系与新选出的恢复系不同，新选出的恢复系UR5-3只能恢复CMSUA5-1，说明UR5-3是一个新型栽培向日葵的恢复系。

Selection of cytoplasmic new male sterile line and restorer line for sunflower

Wild sunflower is important sources in improving cultivated sunflower.Utilizing wild species and cultivated sunflower hybridization to creat new cytoplasmic male sterile line and restorer line is one of the most efficient methods in breeding.After self crossing pollination of selecting plants for multiple hybrid LD06 F1,in F2,plant pairs of fertile and male sterile had been hybridized.Mixing solution of wild species(H.petiolaris) including whole DNA solution and diffused solution had daubed on the pollinated heads at the same time.CMSUA5-1 and maintaining line UB5-2 had been bred successfully through further generation segregation and selection.In the hybrids,tested CMSPET-1’s restore line did not restored CMSUA5-1,which proved that the restorer line differed from CMSPET-1’s restorer line.Restorer line UR5-3 only restored CMSUA5-1,which indicated that this restorer line UR5-3 could be a new restorer line for cultivated sunflower.