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| 小麦抗白粉病基因SRAP标记的鉴定及序列分析 | |
| 引用本文： | 伊艳杰,胡楠,刘红彦,安黎哲,刘新涛,王勋陵.小麦抗白粉病基因SRAP标记的鉴定及序列分析[J].河南农业科学,2007(3):60-62. |
| 作者姓名： | 伊艳杰 胡楠 刘红彦 安黎哲 刘新涛 王勋陵 |
| 作者单位： | 1. 兰州大学,干旱与草地生态教育部重点实验室,甘肃,兰州,730000;河南省农业科学院,植物保护研究所,河南,郑州,450002 2. 南阳理工学院,河南,南阳,473004 3. 河南省农业科学院,植物保护研究所,河南,郑州,450002 4. 兰州大学,干旱与草地生态教育部重点实验室,甘肃,兰州,730000 |
| 基金项目： | 河南省杰出青年科学基金 |
| 摘 要： | 利用240对SRAP引物组合,扩增普通小麦SF42(高感白粉病)和ZB90(对白粉病免疫)。40%的引物组合能在感抗材料中扩增出稳定的多态性条带。进一步用这些引物分析SF42×ZB90的F2分离群体,发现有4对引物组合Me5+Em5,Me8+Em7,Me8+Em16,Me12+Em7扩出的5个SRAP标记与小麦品种ZB90所含的抗白粉病基因连锁。对这些标记进行回收、克隆、测序。序列分析发现,这些标记均含有ORF,并且有4个标记含有信号肽和跨膜区等保守结构域。 |
| 关 键 词： | 小麦 抗白粉病基因 SRAP标记 序列分析 |
| 文章编号： | 1004-3268(2007)03-0060-03 |
| 修稿时间： | 2006-03-08 |
Identification and Analysis of the SRAP Markers Linked with Powdery Mildew Resistance Gene in Wheat |
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| YI Yan-jie,HU Nan,LIU Hong-yan,AN Li-zhe,LIU Xin-tao,WANG Xun-ling.Identification and Analysis of the SRAP Markers Linked with Powdery Mildew Resistance Gene in Wheat[J].Journal of Henan Agricultural Sciences,2007(3):60-62. | |
| Authors: | YI Yan-jie HU Nan LIU Hong-yan AN Li-zhe LIU Xin-tao WANG Xun-ling |
| Institution: | 1. Key Laboratory of Arid and Grassland Ecology of Ministry of Education, Lanzhou University,Lanzhou 730000,China; 2. Henan Academy of Agricultural Sciences,Zhengzhou 450002,China; 3 Nanyang Institute of Technology, Nanyang 473004,China |
| Abstract: | 240 SRAP primer combinations were applied to amplify wheat cultivar SF42(highly susceptible to powdery mildew) and ZB90(immune to powdery mildew).Forty percent of primer combinations could amplify the stable polymorphic bands.Further analyzing the F2 segregating populations from the cross of SF42 and ZB90 using these primers,there are five SRAP markers produced by four primer combinations Me5+Em5、Me8+Em7、Me8+Em16 and Me12+Em7,which were linked with the powdery mildew resistance gene in ZB90.These markers were recovered,cloned and sequenced.Sequence analysis showed these markers contained ORFs and four of ones had conserved motifs such as signal-peptide and transmembrane-regions. |
| Keywords: | Wheat Powdery mildew resistance gene SRAP marker Sequence analysis |
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