不同冻融处理对猪精子顶体膜蛋白表达的影响

为探究不同冷冻解冻方法对猪精子损伤的影响,采用2种常用的冷冻解冻方法处理猪精子,分别对其顶体膜蛋白进行分离,进行SDS-PAGE电泳和总灰度值的检测和分析。结果表明,采用一次稀释法进行猪精液的冷冻保存较二次稀释法解冻后精子活率高,精子顶体膜蛋白组分中除125 ku和90 ku处蛋白表达水平低于二次稀释法外,120 ku、48 ku、36 ku均高于二次稀释法。可见,猪精子在受到更深层次(二次稀释法对精子冷冻解冻损伤大)的冷冻损伤时,伴随着顶体膜蛋白125 ku和90 ku表达水平的升高以及120、48及36 ku表达水平的降低。

Effects of different freezing-thawing treatments on expression of acrosomal membrane proteins in boar sperm

To explore the effects of different frozen-thawed methods on boar sperms, two ways of freeze and thaw the porcine sperms were used. Isolating the sperm acrosomal membrane protein, detecting total proteins by SDS-PAGE electrophoresis, and analyzing the total gray value were conducted. The result showed that the boar sperm viability of freezing and thawing with one-time dilution method was higher than that with two-time dilution method. Compared to one-time dilution method, protein expressions of 125 ku and 90 ku were less than those with two-time dilution method in the components of pig sperm acrosomal membrane proteins, but the protein expressions of 120 ku, 48 ku and 36 ku were higher than those with two-time dilution. In conclusion, there were certain relationships between the boar sperm damage and the expression of acrosomal membrane proteins of 125 ku, 120 ku, 90 ku, 48 ku and 36 ku in the frozen-thawed process of boar sperm.