低温超低温对棉花花粉活力的影响

通过使用联苯胺-甲萘酚染色法测定4、-20和-80℃下棉花花粉活力,研究低温对花粉活力的影响,结果表明:离体花粉在4和-20℃的条件下1～3 d内花粉活力变化没显著差异.在-80℃下,花粉活力受到低温预处理和蔗糖溶液浓度(用作冷冻保护剂)的交互作用影响.并且低温预处理后的花粉活力维持在较高水平.同时,没有添加冷冻保护剂的花粉活力与使用30;和20;浓度的冷冻保护剂之间并没有显著差异.在化冻方式上,37℃的水浴中化冻效果最好.

Impact of Low Temperature and Ultra -low Temperature on the Cotton Pollen Viability

In order to study the impact of the low temperature on cotton pollen viability,pollen viability were detected using Benzidine -α- Naphthol coloring under 4℃,- 20℃and - 80℃.The results showed:there was no significant change of pollen viability after storing for 1 - 3 days at 4℃and - 20℃.Under storing at- 80℃,the pollen viability showed interaction effects of low - temperature pretreatment and sucrose concentration (Sucrose solution used as cryoprotectant).Pollen viability remained at higher level after low - temperature pretreatment.There are no significant differences between pollen viability without adding cryoprotectants and pollen viability with cryoprotectants of 30%and 20%concentration.And,the best thaw method is at 37℃in a water bath.