氧氟沙星在乌骨鸡体内残留消除规律研究

采用高效液相色谱法分析氧氟沙星在乌骨鸡体内各组织中的残留消除规律。荧光检测器的激发波长为280nm，发射波长为450Dill。色谱柱为ttypersilODS—C18，柱温40~C，以磷酸（以三乙胺调pH至2．5）-乙腈（82：18）为流动相，流速1．0mL／min，进样量为20肛L。氧氟沙星在乌骨鸡肌肉、肝脏、。肾脏中的平均回收率分别为86．3％、83．2％、82．5％。检测限为10ng／g。当组织中氧氟沙星浓度在10～2000ug／kg范围时，线性关系良好。利用该方法对氧氟沙星在乌骨鸡体内的残留消除规律进行了研究，结果表明，氧氟沙星在鸟骨鸡体内的残留消除速度极为缓慢。40天休药期后，2日龄使用组可在肌肉、肝脏和肾脏中分别检出氧氟沙星146．8“g／kg、20．6“g／kg和437．4μg／kg；28日龄使用组分别检测出193．5ug／kg、45．0μg／kg和425．5μg／kg。2日龄使用组休药78天时（即停药71天），肌肉和肝脏中还能分别检出氧氟沙星11．2μg／kg和12．0μg／kg。实验表明乌骨鸡使用氧氟沙星仅适用于10日龄以下给药并休药70天以上，或不给予该类药物。

Studies on residues of Ofloxacin in black-bone silky fowl

A reversed-phase high performance liquid chromatographic method has been developed for the determination of ofloxacin residues in black-bone silky fowl.After being extracted with phosphate buffer, ofloxacin was analyzed by HPLC(Hypersil BDS-C18) and detected by FLD(λex= 280 nm,λem = 450 tun). The mobile phase was acetonitrile-phosphoric acid/triethylamine(18:82).The linear range was10～2 000μg/kg .The average recovery of ofloxacin was 86.3%,83.2%or 82.5%in muscle,liver or kindney respectively.The minimal detectable concentration of ofloxacin was 10 ng/g.Results of this experiment showed that the depletion of ofloxacin in muscle and kidney was slow.After 40 days of withdrawal,the concentration of ofloxacin in the chickens used at 2 days old were 146.8μg/kg,20.6μg/kg or 437.4μg/kg in muscle,liver and kidney respectively,The concentration of ofloxacin in the chickens used at 28 days old were 193.5μg/kg,45.0μg/kg or 425.5μg/kg respectively.After 71 days of withdrawal,the concentration of ofloxacin in the chickens used at 2 days old were 11.2μg/kg or 12μg/kg in the muscle and liver respectively.This experiment showed that Ofloxacin should only be applied to the chickens younger than 10 days old with more than 70 days of withdrawal or should not be used in raising.