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| 刺五加成熟种子的愈伤组织诱导及其RAPD分析 | |
| 引用本文： | 邢朝斌,沈海龙,刘岩,罗玲,范少辉. 刺五加成熟种子的愈伤组织诱导及其RAPD分析[J]. 种子, 2006, 25(7): 44-45,49 |
| 作者姓名： | 邢朝斌 沈海龙 刘岩 罗玲 范少辉 |
| 作者单位： | 1. 华北煤炭医学院生物科学系,河北,唐山,063000 2. 东北林业大学林学院,黑龙江,哈尔滨,150040 3. 中国林业科学研究院林业研究所,北京,100091 |
| 摘 要： | 以刺五加的成熟种子为材料，通过离体培养诱导产生愈伤组织，利用RAPD分子标记方法，在DNA水平上分析无菌苗和愈伤组织的遗传变异。10个10碱基引物中筛选出4个引物，对它们的PCR扩增结果进行了分析。结果表明刺五加愈伤组织与无菌苗相比出现了DNA水平的变异。 |
| 关 键 词： | 刺五加 愈伤组织 |
| 收稿时间： | 2006-04-06 |
| 修稿时间： | 2006-04-06 |
Induction of Callus of Eleutherococcus senticosus Ripe Seeds and Its RAPD Analysis |
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| Xing Zhaobin,Shen Hailong,Liu Yan,Luo Ling,Fan Shaohui. Induction of Callus of Eleutherococcus senticosus Ripe Seeds and Its RAPD Analysis[J]. Seed, 2006, 25(7): 44-45,49 | |
| Authors: | Xing Zhaobin Shen Hailong Liu Yan Luo Ling Fan Shaohui |
| Affiliation: | 1. Department of Biology, North China Coal Medical College ,Tangshan 063000 ; 2. College of Forestry, Northeast Forestry University, Harbin, 150040 ; 3. Research institute of Forestry, CAF, Beijing 100091 |
| Abstract: | Eleutherococcus senticosus were used for inducation of callus in vitro.Genetic differences between aseptic plantlets and callus had been analysed on DNA level with the application of random amplified polymorphic DNA(RAPD) molecular marker.The fingerprints amplification by polymerase chain reaction(PCR) with 4 polymorphic 10-based random primers selected from 10 ones was analysised.The results indicated that there were genetic variation between aseptic plantlet and callus. |
| Keywords: | RAPD |
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