嗜热脂肪芽孢杆菌检测牛乳中抗生素残留的研究

基于嗜热脂肪芽孢杆菌繁殖分解乳糖产酸,将其应用于原料乳的抗生素残留检测。以嗜热脂肪芽孢杆菌为指示菌株,通过改变指示剂组成、添加增效剂乳糖酶以及对分析条件进行筛选,得到适宜的检测条件参数。具体条件参数为:菌液活菌数5×106,菌液添加量0.1mL,30g·L-1的乳糖酶溶液0.1mL,混合指示剂0.1mL,乳样0.1mL,发酵温度64℃,检测时间2.5h。与国家标准法(TTC法)相比较,所研究的方法对5种抗生素的检出限更低,结果更易于肉眼判断。

Study on the application of Bacillus stearothermophilus in the detection of antibiotics residues in milk

Based on the fact that Bacillus stearothermophilus can degrade lactose to produce acid, Bacillus stearothermophilus was used as indicator strain to detect antibiotics residues in the raw milk. The composition of mixed indicators was modified and lactase was added to milk sample to enhance detection rate. Other detection parameters were also studied. The optimal parameters for detection were as follows: the viable cells of Bacillus stearothermophilus 5×10~6 in 0.1 mL solution, addition of 30 g·L~(-1) lactase solution 0.1 mL, addition volume of indicator 0.1 mL, detection volume of milk 0.1 mL, fermentation temperature 64°C and detection time 2.5 h. Compared with national standard method (TTC method), this new method has lower detection limits for five antibiotics, and is easy to judge result by nude eyes.