| 百合根尖染色体制片技术研究 |
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| 引用本文： | 荣立苹,雷家军,王志刚.百合根尖染色体制片技术研究[J].安徽农业科学,2007,35(7):2006-2006,2009. |
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| 作者姓名： | 荣立苹 雷家军 王志刚 |
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| 作者单位： | 沈阳农业大学园艺学院,辽宁沈阳,110161;辽宁省农业科学院花卉研究所,辽宁沈阳,110161 |
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| 摘 要： | 以卷丹百合根尖为试材,对其染色体制片技术的取样时间、预处理液、解离时间、染色剂等方面进行了试验研究,并对3个居群的卷丹百合染色体数目进行了观察。结果表明,根尖取样时间以上午8:30~9:30为佳,用0.1%秋水仙碱溶液4℃下预处理24小时,经卡诺固定液固定6h后,在1mol/L盐酸60℃下恒温水浴中解离8 min,改良卡宝品红溶液染色压片镜检,能取得良好的制片效果。卷丹染色体数目观察表明,3个卷丹居群的染色体数目均为2n=3x=36。
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| 关 键 词： | 卷丹 染色体 制片技术 |
| 文章编号： | 0517-6611（2007）07-02006-01 |
| 修稿时间： | 2006-12-06 |
Studies on Technique of Chromosome Squashing of Root-tip in Lilium lancifolium Thtmb |
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| RONG Li-ping et al.Studies on Technique of Chromosome Squashing of Root-tip in Lilium lancifolium Thtmb[J].Journal of Anhui Agricultural Sciences,2007,35(7):2006-2006,2009. |
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| Authors: | RONG Li-ping |
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| Institution: | College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110161 |
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| Abstract: | The roots of L.lancifolium Thunb.were used to study the effects of the sampling time,the pretreatment chemical solution,hydrolysing time and staining on the chromosome squashing.The results showed that the optimal sampling time of roots was about 8:30～9:30 am.Among the three pretreatment of chemical solutions,0.1% colchicine solution got best result.First,0.1% colchicine solution was pretreated at 4 ℃ for 24 h,then the root-tips were fixed with Carnoy solution for 6 h,hydrolysed with 1 mol/L HCl for 8 min at 60 ℃ and stained with modified Carbol fuchsin solution.With this method a very good effect of chromosome squashing could be obtained.The observation indicated that the chromosome number of 3 populations of Lilium lancifolium was 2n=3x=36. |
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| Keywords: | L lancifolium Thunb Chromosome Technique of squashing |
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