| 玉米根围土壤中腐霉菌的分离鉴定及核糖体DNA-ITS序列分析 |
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| 引用本文： | 柴兆祥,李金花,楼兵干,李唯,甘辉林,郭成,赵玲,董迪.玉米根围土壤中腐霉菌的分离鉴定及核糖体DNA-ITS序列分析[J].草业学报,2009,18(3):126-135. |
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| 作者姓名： | 柴兆祥 李金花 楼兵干 李唯 甘辉林 郭成 赵玲 董迪 |
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| 作者单位： | 1.甘肃农业大学草业学院,甘肃 兰州 730070;2.甘肃省作物遗传改良与种质创新重点实验室,甘肃 兰州 730070;3.草业生态系统教育部重点实验室,甘肃 兰州 730070;4.中-美草地畜牧业可持续发展研究中心,甘肃 兰州 730070;5.浙江大学生物技术研究所,浙江 杭州 310029;6.甘肃农业大学生命科学技术学院,甘肃 兰州 730070 |
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| 基金项目： | 甘肃省科学事业费项目，甘肃省教育厅项目 |
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| 摘 要： | 从兰州地区的红古、七里河等地采集玉米根围土壤标样,诱饵法与组织分离法相结合分离纯化到23株腐霉菌株,通过形态特征、培养特性、生长速度和对温度适应范围的研究,结合rDNA-ITS序列分析,将分离到的腐霉菌株鉴定为硬腐霉、终极腐霉和一个腐霉待定种。这是首次报道在我国分离到硬腐霉,为我国新记录种;终极腐霉也系首次在甘肃从玉米根围土壤中分离到。统计表明,3种腐霉菌种在兰州地区玉米根围土壤中的分离频率不同,终极腐霉出现次数最多,为优势种群,分离频率为60.9%;其次为腐霉待定种,分离频率为26.1%;而硬腐霉分离频率最低,为13.0%。
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| 关 键 词： | 玉米 根围土壤 腐霉 鉴定 核糖体DNA-ITS |
| 收稿时间： | 2008-08-16 |
Isolation,identification,and sequence analysis of rDNA-ITS of Pythium species sampled from rhizosphere soil of corn |
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| CHAI Zhao-xiang,LI Jin-hua,LOU Bing-gan,LI Wei,GAN Hui-lin,GUO Cheng,ZHAO Ling,DONG Di.Isolation,identification,and sequence analysis of rDNA-ITS of Pythium species sampled from rhizosphere soil of corn[J].Acta Prataculturae Sinica,2009,18(3):126-135. |
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| Authors: | CHAI Zhao-xiang LI Jin-hua LOU Bing-gan LI Wei GAN Hui-lin GUO Cheng ZHAO Ling DONG Di |
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| Institution: | CHAI Zhao-xiang1,2,3,4,LI Jin-hua1,LOU Bing-gan5,LI Wei6,GAN Hui-lin 1,GUO Cheng1,ZHAO Ling1,DONG Di1(1.College of Grassl, Sciences,Gansu Agricultural University,Lanzhou 730070,China,2.Gansu Key Lab of Crop improvement , Germplasm Enhancement,3.Key Laboratory of Grassl, Ecosystem,Ministry of Education,4.Sino-U.S.Centers for Grazingl, Ecosystem Sustainability,5.Institute of Biotechnology,Zhejiang University,Hangzhou 310029,6.Colle... |
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| Abstract: | Pythium spp.were isolated from the rhizosphere soils of corn sampled from Honggu and Qilihe districts of Lanzhou using a baiting method.Twenty three isolates were obtained after subculture and purification.Based on the morphology,culture characteristics,growth rate,adaptation to temperatures and sequence analysis of rDNA-ITS,the 23 isolates were all identified as P.scleroteichum,P.ultimum or Pythium sp.This is the first report of P.scleroteichum in China and of P.ultimum from corn in Gansu.P.ultimum was the... |
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| Keywords: | Zea mays rhizosphere soil Pythium spp identification rDNA-ITS |
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