| 空间诱变辣椒AFLP反应体系的优化 |
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| 引用本文： | 周春阳,郭亚华,谢立波,王雪,高永利,周宇,黄凤兰,彭木.空间诱变辣椒AFLP反应体系的优化[J].北方园艺,2011(21):103-105. |
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| 作者姓名： | 周春阳 郭亚华 谢立波 王雪 高永利 周宇 黄凤兰 彭木 |
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| 作者单位： | 东北农业大学园艺学院,黑龙江哈尔滨,150030;黑龙江省农业科学院园艺分院,黑龙江哈尔滨,150069;内蒙古民族大学生命科学学院 |
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| 基金项目： | 航天育种工程资助项目(2006HT20012); 国家大宗蔬菜产业技术体系资助项目(CARS-25-G-11); 黑龙江省自然科学基金重点资助项目(ZD200818-01),黑龙江省自然科学基金资助项目(C200943) |
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| 摘 要： | 为了建立适合空间诱变辣椒的AFLP反应体系,以3～4叶期的辣椒叶片为试材,对体系中的DNA用量、酶切与连接、预扩增及产物的稀释倍数等关键因素进行了优化,确立了适合空间诱变辣椒的AFLP反应体系.结果表明:采用该体系对空间诱变辣椒进行AFLP分析,能够得到清晰稳定的分子标记图谱.
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| 关 键 词： | 空间诱变 辣椒 AFLP反应体系 优化 |
Optimization of AFLP System for Hot Pepper Induced by Space |
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| ZHOU Chun-yang
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GUO Ya-hua
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XIE Li-bo
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WANG Xue
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GAO Yong-li
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ZHOU Yu
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HUANG Feng-lan
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PENG Mu.Optimization of AFLP System for Hot Pepper Induced by Space[J].Northern Horticulture,2011(21):103-105. |
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| Authors: | ZHOU Chun-yang GUO Ya-hua XIE Li-bo WANG Xue GAO Yong-li ZHOU Yu HUANG Feng-lan PENG Mu |
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| Institution: | ZHOU Chun-yang1,GUO Ya-hua2,XIE Li-bo2,WANG Xue2,GAO Yong-li2,ZHOU Yu2,HUANG Feng-lan3,PENG Mu3(1.College of Horticulture,Northeast Agricultural University,Harbin,Heilongjiang 150030,2.Horticultural Sub-Academy,Heilongjiang Academy of Agricultural Sciences,Heilongjiang 150069,3.College of Life Sciences,Inner Mongolia University for the Nationality,Tongliao,Inner Mongolia 028000) |
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| Abstract: | In order to establish a suitable AFLP reaction system of pepper induced by space,taking 3~4 leaf stage of pepper leaves as test materials,several significant influencing factors,including the dosage of template DNA,enzyme digestion and ligation,pre-amplification and the dilute of pre-amplification products were optimized,and a suitable AFLP reaction system for hot pepper induced by space was constructed.The results showed that by this optimized system,a clear and credible AFLP fingerprint could be obtained. |
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| Keywords: | induced by space hot pepper AFLP analysis system optimization |
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