| 猪多潜能性维持因子5 cDNA全序列的克隆与分析 |
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| 引用本文： | 李勇,王立刚,王立贤,乔丽娟,张龙超,颜华.猪多潜能性维持因子5 cDNA全序列的克隆与分析[J].农业生物技术学报,2010,18(2):285-289. |
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| 作者姓名： | 李勇 王立刚 王立贤 乔丽娟 张龙超 颜华 |
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| 作者单位： | 中国农业科学院北京畜牧兽医研究所,北京,100193 |
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| 基金项目： | 国家科技支撑计划，国家高技术研究与发展计划(863) |
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| 摘 要： | 利用前期筛选出的一些与母猪妊娠相关的有价值的新候选基因,选取其中一个位于网络顶端的EST序列(CK455976),根据此EST序列设计引物,以母猪卵巢为材料提取总RNA,运用RT-PCR和RACE-PCR对其进行cDNA全序列克隆,得到多潜能性维持因子5(developmental pluripotency associated 5,DPPA5)基因cDNA全长596 bp,提交GenBank数据库,登录号为FJ436413.经序列分析发现,其中5'UTR长67 bp,3'UTR长175 bp,编码区长354 bp,编码117个氨基酸.同源性比对分析发现,该基因核苷酸序列与人、小鼠、猕猴和狗的DPPA5基因同源性分别为83%、70%、83%和80%;编码的氨基酸序列同源性分别为74%、55%、74%和69%.经生物信息学分析,预测出DPPA5的相对分子量约13.60 kD,等电点5.24,在约1～10、18～29、38～51、60～66和75～110位之间氨基酸存在亲水性区域,DPPA5蛋白主要存在于细胞膜外侧.
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| 关 键 词： | 猪 多潜能性维持因子5 序列克隆 生物信息学分析 |
Porcine Developmental Pluripotency Associated 5 Gene Complete cDNA Cloning and its Bioinformatics Analysis |
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| Li Yong,Wang Ligang,Wang Lixian,Qiao Lijuan,Zhang Longchao,Yan Hua.Porcine Developmental Pluripotency Associated 5 Gene Complete cDNA Cloning and its Bioinformatics Analysis[J].Journal of Agricultural Biotechnology,2010,18(2):285-289. |
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| Authors: | Li Yong Wang Ligang Wang Lixian Qiao Lijuan Zhang Longchao Yan Hua |
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| Institution: | Li Yong Wang Ligang Wang Lixian Qiao Lijuan Zhang Longchao Yan Hua Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing,100193 |
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| Abstract: | An expressed sequence tag(EST, CK455976) on the top of sow gestation relevant gene regulatory networks which was constructed by former reacher in author's laboratory was selected to do forward research in this paper.Using the total RNA extracted from sow ovaries and primers designed according to the sequence of this EST, cDNA complete sequence of the developmental pluripotency associated 5 was obtained using RT-PCR and RACE-PC, the length of this sequence was 596 bp and the accession number was FJ36413 in G... |
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| Keywords: | Pig Developmental pluripotency associated 5 gene Cloning the sequences Bioinformatics analyses |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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