|
|||||
|
|
Efficient shoot regeneration and somatic embryogenesis from immature cotyledons of Brassica napus L. |
|
| Authors: | K. Turgut M. Barghchi R. Scott |
| Affiliation: | Department of Field Crops, Faculty of Agriculture, Akdeniz University, PO Box 510, 07070 Antalya, Turkey;Department of Applied Biology and Biotechnology, The David Attenborough Laboratories, De Montfort University, Scraptoft, Leicester LE7 9SU, UK;Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK |
| Abstract: | Immature cotyledons of Brassica napus cv.‘Topas’ were used to investigate the effects of various naphthaleneacetic acid and benzylaminopurine concentrations on morphogenesis in vitro. A high efficiency of adventitious shoot regeneration and somatic embryogenesis was achieved on Murashige and Skoog medium supplemented with the above growth regulators. Two types of somatic embryogenesis were obtained, the first directly and the second via a callus stage. |
| Keywords: | Brassica napus immature cotyledon shoot regeneration somatic embryogenesis |