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茄根中总多酚含量的测定
引用本文:李双双,李维双,代小丽,周媛. 茄根中总多酚含量的测定[J]. 安徽农业科学, 2010, 38(30): 16855-16857. DOI: 10.3969/j.issn.0517-6611.2010.30.056
作者姓名:李双双  李维双  代小丽  周媛
作者单位:三峡大学天然产物研究与利用湖北省重点实验室,湖北宜昌,443002;三峡大学天然产物研究与利用湖北省重点实验室,湖北宜昌,443002;三峡大学天然产物研究与利用湖北省重点实验室,湖北宜昌,443002;三峡大学天然产物研究与利用湖北省重点实验室,湖北宜昌,443002
基金项目:湖北省自然科学基金创新群体计划
摘    要:采用改进的Folin-Ciocalteu比色法测定茄根中总多酚的含量。结果表明,对照品标准曲线在0.0096~0.0768mg线性关系良好。茄根的75%乙醇提取物中总多酚的平均含量为0.272%,RSD为0.98%;平均加标回收率为97.47%,RSD为3.64%。

关 键 词:茄根  总多酚  Folin-Ciocalteu比色法

Content Determination on Total Polyphenol in Eggplant Root
Affiliation:LI Shuang-shuang et al (Hubei Provincial Key Laboratory for Natural Products Research and Utilization,Three Gorge's University,Yichang,Hubei 443002)
Abstract:The content of total polyphenol in eggplant root was determined by using improved Folin-Ciocalteu colorimetry.The results showed that in the range of 0.009 6-0.076 8 mg,the linear relationship of standard curve of reference substance was good.The average content of total polyphenol in 75% alcohol extract from eggplant root was 0.272% and its RSD was 0.98%.The average recovery of standard addition of this method and its RSD were 97.47% and 3.64% resp.
Keywords:Eggplant root   Total polyphenol   Folin-Ciocalteu colorimetry
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