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| 国槐组织培养生根的研究 | |
| 引用本文： | 刘桂民,燕丽萍,尹国良,李自成,刘翠兰,孙超,夏阳.国槐组织培养生根的研究[J].山东林业科技,2012,42(4):42-44,4. |
| 作者姓名： | 刘桂民 燕丽萍 尹国良 李自成 刘翠兰 孙超 夏阳 |
| 作者单位： | 1. 山东省林业科学研究院,山东济南,250014 2. 山东省林业科学研究院,山东济南250014 山东省林木遗传改良重点实验室 3. 寿光市机械林场 |
| 基金项目： | 山东省良种工程农业生物资源创新利用研究 |
| 摘 要： | 通过不同基本培养基、植物生长调节剂IAA、NAA、IBA和活性炭等对国槐试管苗不定根的诱导进行了系统研究。筛选出国槐最佳生根培养基是1/2MS+IAA0.5mg/L+蔗糖2%+琼脂0.65%,生根率达86%以上;并对不同苗龄试管苗不定根的发育过程进行了研究,结果表明,苗龄为20～25天的试管苗生根率最高可达90%,而且生根周期较短。 |
| 关 键 词： | 国槐 外源生长素类 不定根 组织培养 |
Study on Rooting in vitro shoots of the Sophora japonica L |
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| Yan Liping,Yin Guoliang,Li Zicheng,Liu Cuilan,Sun Chao,Xia Yang.Study on Rooting in vitro shoots of the Sophora japonica L[J].Journal of Shandong Forestry Science and Technology,2012,42(4):42-44,4. | |
| Authors: | Yan Liping Yin Guoliang Li Zicheng Liu Cuilan Sun Chao Xia Yang |
| Institution: | 1. Shandong Provincial Academy of Forestry, Jinan 250014, China ; 2. Shandong Provincial Key Laboratory of Forest Tree Genetic Improvement) |
| Abstract: | The effect of the different basic medium, IAA,NAA,IBA and active carbon on rooting of shoots in test tubes of the Sophora japonica L was studied , The optimal medium of rooting is 1/2MS@ IAA 0.5mg/L @sucrose 2% fro @agar 0.65%, rooting ratio would reach 86%. The growth process of adventitious root in different seedling age was also studied,he result in dicated that the root rate of shoots in test tubes which the seedling age was 20%25day--long reached 90%, moreover the root cycle was short. |
| Keywords: | Sophorajaponica L exogenous auxins adventitious root tissue culture |
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