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| PVY株系间的分子变异及分子鉴定方法 | |
| 引用本文： | 杨庆东,吴兴泉,陈士华,刘晓磊. PVY株系间的分子变异及分子鉴定方法[J]. 中国马铃薯, 2011, 25(3): 166-169 |
| 作者姓名： | 杨庆东 吴兴泉 陈士华 刘晓磊 |
| 作者单位： | 1. 黑龙江八一农垦大学,黑龙江大庆,163319 2. 河南工业大学生物工程学院,河南郑州,450001 |
| 基金项目： | 河南省教育厅自然科学基础研究项目(2006210003); 河南工业大学校科研基金项目(07XJC008) |
| 摘 要： | 马铃薯Y病毒(Potato virus Y,PVY)株系分化现象明显,难于准确鉴定.本研究利用核酸序列分析工具对PVY主要株系的全基因组序列、cp基因序列和P1基因序列进行了系统分析,探明了PVY主要株系的分子特征.结果表明,具有PVYN株系血清型的PVYN、PVYNIN株系与具有PVYO株系血清型的PVYO、PVYN... |
| 关 键 词： | 马铃薯Y病毒 株系 分子变异 分子鉴定 |
Molecular Variation and Identification of Potato Virus Y Strains |
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| YANG Qingdong,WU Xingquan,CHEN Shihua,LIU Xiaolei. Molecular Variation and Identification of Potato Virus Y Strains[J]. Chinese Potato, 2011, 25(3): 166-169 | |
| Authors: | YANG Qingdong WU Xingquan CHEN Shihua LIU Xiaolei |
| Affiliation: | YANG Qingdong1,WU Xingquan2,CHEN Shihua2,LIU Xiaolei2(1.Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China,2.College of Biotechnology,Henan University of Technology,Zhengzhou,Henan 450001,China) |
| Abstract: | Strain differentiation of potato virus Y(PVY) is obvious,and it is difficult to be identified.The complete genome,cp and p1 genes of different PVY strains were analyzed with informatics tools,and the molecular variation characters among different PVY strains were proved up.It was confirmed that the nucleotide at 50 nt sites and 86 nt sites in cp gene was different among the strains which belong to PVYN serotype(PVYN,PVYNTN) and PVYO serotype(PVYO,PVYN : O,and PVYNW).It was confirmed that the PVY strains cou... |
| Keywords: | potato virus Y strain molecular variation molecular identification |
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